T8203

Anti-TUBA1A (TUBA3) Antibody

Name: Anti-TUBA1A (TUBA3) Antibody
Brand: SIGMA

mouse monoclonal, AA13

Synonym(s):

Anti-Tuba1a, Anti-Tubulin, α1A

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00145891

Product Name

Anti-α-Tubulin antibody, Mouse monoclonal, clone AA13, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AA13, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

human, rat, mouse

packaging

antibody small pack of 25 μL

enhanced validation

independent
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concentration

~2.0 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using Neuro-2a cell extract

isotype

IgG1

UniProt accession no.

Q71U36

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Application

Anti-α-Tubulin antibody, Mouse monoclonal has been used in western blotting.

The antibody has been used in various immunochemical techniques including immunoprecipitation, immunocytochemistry, and immunohistochemistry.

Biochem/physiol Actions

Gene mutation of tubulin α-1A (TUBA1A) is observed in perisylvian asymmetrical polymicrogyria, polymicrogyria-like cortical dysplasia, and microlissencephaly in foetal cases. Alteration in the expression of the gene impairs neural migration and causes lissencephaly (LIS).

Tubulin is the major building block of microtubules, which is a structural and mobile element in mitosis, intracellular transport, flagellar movement and the cytoskeleton. Monoclonal antibodies recognizing α-tubulin, together with monoclonal antibodies to other tubulin types (α-tubulin isotypes I, II, and III, tyrosine tubulin and acetylated- α -tubulin) provide a specific and useful tool in studying the intracellular distribution of tubulin, and the static and dynamic aspects of the cytoskeleton. In addition, the various tubulin forms have been implicated in cancerous processes and are thus suggested as potential targets for cancer therapy.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-α-Tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma AA13 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with rat brain tubulin.

Tubulin α 1a (TUBA1A) is a member of α-tubulin gene family. It is encoded by the gene mapped to human chromosome 12q13.12. Tubulin α 1a is a vital structural subunit of microtubules that are temporarily expressed in almost all post-mitotic neurons during neuronal development.
The encoded protein structure contains core of two β-sheets enclosed by α-helices. TUBA1A is characterized with an amino-terminal domain with the guanine nucleotide-binding region, an intermediate domain associated with interprotofilament lateral contacts and the modulation of tubulin curvature, and a carboxy-terminal domain that contains region involved in interacting with microtubule-associated proteins (MAPs) and molecular motors, such as kinesins and dynein.

Tubulin is a cylindrical, filamentous structure is present inalmost all eukaryotic cells.

Immunogen

rat brain tubulin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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New tubulin targeting agents currently in clinical development

Carlson RO

Expert Opinion on Investigational Drugs, 17(5), 707-722 (2008)

Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress.

Ahlskog JK

Molecular and Cellular Biology, 30(24), 5608-5620 (2010)

TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis.

Yokoi S

Scientific Reports, 5:15165 (2015)

A case of TUBA1A mutation presenting with lissencephaly and Hirschsprung disease.

Brain & Development, 36(2), 159-162 (2014)

Expanding the spectrum of TUBA1A-related cortical dysgenesis to Polymicrogyria.

Poirier K

European Journal of Human Genetics, 21(4), 381-385 (2013)

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