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Merck

T8768

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate

horseradish peroxidase substrate, chromogenic, ≥98%, powder

Synonym(s):

TMB dihydrochloride

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About This Item

Linear Formula:
[-C6H2(CH3)2-4-NH2]2·2HCl·xH2O
CAS Number:
207738-08-7
Molecular Weight:
313.27 (anhydrous basis)
UNSPSC Code:
12352204
PubChem Substance ID:
24900558
NACRES:
NA.83
MDL number:
MFCD00150104

Product Name

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate, powder, ≥98%

SMILES string

O.Cl.Cl.Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI key

KVCWTKDFVVSVSJ-UHFFFAOYSA-N

InChI

1S/C16H20N2.2ClH.H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;;/h5-8H,17-18H2,1-4H3;2*1H;1H2

assay

≥98%

form

powder

solubility

acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy

storage temp.

2-8°C

Quality Level

200

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Application

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).
Noncarcinogenic substitute for benzidine.

General description

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000397861
0000381324
0000345095
0000324604
0000293488

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Lymphocyte Homing to the Skin: Immunology, Immunopathology, and Therapeutic Perspectives (2004)
Detection of Antibodies Against DNA Polymerase of Hepatitis B Virus in HBsAg-Positive Sera Using ELISA
Rui LX, et al.
The Korean Journal of Internal Medicine, 13, 95-95 (1998)
Robot Printing of Reverse Dot Blot Arrays for Human Mutation Detection
Lappin S, et al.
The Journal of Molecular Diagnostics : JMD, 3, 178-178 (2001)
Quantitative determination of hemoglobin and cytochemical staining for peroxidase using 3, 3?, 5, 5?-tetramethylbenzidine dihydrochloride, a safe substitute for benzidine
Liem HH, et al.
Analytical biochemistry, 98(2), 388-393 (1979)
R Piovesana et al.
Scientific reports, 10(1), 7159-7159 (2020-04-30)
Regenerative capability of the peripheral nervous system after injury is enhanced by Schwann cells (SCs) producing several growth factors. The clinical use of SCs in nerve regeneration strategies is hindered by the necessity of removing a healthy nerve to obtain
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