T8823

Monoclonal Anti-T7 tag antibody produced in mouse

Name: Monoclonal Anti-T7 tag antibody produced in mouse
Brand: SIGMA

purified immunoglobulin, buffered aqueous solution

Synonym(s):

T7 Tag antibody

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.56

MDL number:

MFCD02689159

Product Name

Monoclonal Anti-T7 tag antibody produced in mouse, purified immunoglobulin, buffered aqueous solution

isotype

IgM

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

T7 Tag

form

buffered aqueous solution

concentration

~2 mg/mL

technique(s)

western blot (chemiluminescent): 1-2 μg/mL using recombinant T7-tagged β-galactosidase

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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Analysis Note

Antibody is suitable for ELISA and immunoblotting.

Application

Monoclonal Anti-T7 tag antibody produced in mouse has been used in western blotting.

General description

The Mouse monoclonal Anti-T7 tag antibody recognizes the T7 tag sequence on T7-tagged fusion proteins. The T7 tag functions as a marker in many expression vectors.

The T7 tag is a 11 amino acid peptide, encoded in the leader sequence of T7 bacteriophage gene 10. This gene encodes a T7 major capsid protein.

Immunogen

synthetic peptide corresponding to the T7-tag (MASMTGGQQMG-K), conjugated to KLH

Physical form

Solution at approximately 2 mg/ml in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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ESSENTIALS OF MEDICINAL CHEMISTRY (2013)

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DNA methyltransferases make use of alternative splicing mechanism to generate various splice variants, but their role(s) in modulating DNA methylation patterns in the cells is unclear. Notably, DNMT3B alone contains nearly 40 different splice variants. In this study, we have

Design of high-throughput methods of protein production for structural biology

Stevens RC

Structure, 8(9), R177-R185 (2000)

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Test, 291(49), 25591-25607 (2016)

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