T9028

Monoclonal Anti-Tubulin, Tyrosine antibody produced in mouse

Name: Monoclonal Anti-Tubulin, Tyrosine antibody produced in mouse
Brand: SIGMA

clone TUB-1A2, ascites fluid

Synonym(s):

Anti-CDCBM6, Anti-CSCSC1, Anti-M40, Anti-OK/SW-cl.56, Anti-TUBB1, Anti-TUBB5

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All Photos(3)

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164753

Product Name

Monoclonal Anti-Tubulin, Tyrosine antibody produced in mouse, clone TUB-1A2, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

TUB-1A2, monoclonal

contains

15 mM sodium azide

species reactivity

human, plant, animal

technique(s)

indirect immunofluorescence: 1:800 using cultured chicken fibroblasts
microarray: suitable
western blot: suitable

isotype

IgG3

UniProt accession no.

P07437

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TUBA4A(7277), TUBB(203068)

Application

Monoclonal Anti-Tyrosine Tubulin has been used in:

indirect immunofluorescent labelling
immunoblotting technique
immunocytochemical staining

Biochem/physiol Actions

The antibody reacts against tubulin′s C-terminal tyrosine in immunoblotting assays and may be used for localization of this epitope in cultured cells or tissue sections.

Tubulin acts as a major building block of microtubules. Tubulin tyrosinylation is involved in the assembly status of tubulin. A specific tubulinyl tyrosine carboxypeptidase removes the terminal tyrosine to yield an α-tubulin terminating in a glutamic acid residue while another enzyme modifies the α-tubulin by addition of tyrosine to the carboxy terminus to offer a potential cycle of tyrosine addition and loss.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Tyrosine Tubulin (mouse IgG3 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin as a cylindrical filamentous structure and is present in almost all eukaryotic cells. Tubulin is a heterodimer which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology.

The intracellular cylindrical filamentous structure, microtubules is mainly made up of tubulin and is present in all eukaryotic cells. Monoclonal Anti-Tyrosine antibody can be used in immunocytochemical localization of tyrosinated α tubulin by indirect immunofluorescence labelling. Monoclonal Anti-Tyrosine antibody reacts specifically with tyrosine tubulin of bovine brain, African Green Monkey kidney cells (Vero), dog kidney (MDCK), marsupial kidney (Potoroo, PtK2), mouse pituitary tumour (AtT-20), yeast, and Xenopus.

Immunogen

peptide containing the carboxy-terminal amino acids of α-tubulin

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number

0000512418

0000493501

0000478548

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Evolutionary origin of gastrulation: insights from sponge development.

Nagayasu Nakanishi et al.

BMC biology, 12, 26-26 (2014-04-01)

The evolutionary origin of gastrulation--defined as a morphogenetic event that leads to the establishment of germ layers--remains a vexing question. Central to this debate is the evolutionary relationship between the cell layers of sponges (poriferans) and eumetazoan germ layers. Despite

A marginal band of microtubules transports and organizes mitochondria in retinal bipolar synaptic terminals.

Malkolm Graffe et al.

The Journal of general physiology, 146(1), 109-117 (2015-07-01)

A set of bipolar cells in the retina of goldfish contains giant synaptic terminals that can be over 10 µm in diameter. Hundreds of thousands of synaptic vesicles fill these terminals and engage in continuous rounds of exocytosis. How the

Mechanism of colchicine-induced steroidogenesis in rat adrenocortical cells.

L J Lee et al.

Journal of cellular biochemistry, 81(1), 162-171 (2001-02-17)

Conflicting data for the effects of colchicine on cholesterol transport and steroidogenesis raise the question of the role of microtubules in cholesterol transport from the lipid droplet to mitochondria in steroidogenic cells. In this study, using corticosterone radioimmunoassay and immunofluorescence

Microtubule Structures

Molecular Cell Biology. 4th edition. (2000)

The ontogeny of choanocyte chambers during metamorphosis in the demosponge Amphimedon queenslandica.

Shunsuke Sogabe et al.

EvoDevo, 7, 6-6 (2016-03-10)

The aquiferous body plan of poriferans revolves around internal chambers comprised of choanocytes, a cell type structurally similar to choanoflagellates. These choanocyte chambers perform a range of physiological and developmental functions, including the capture of food and the generation of

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