V3638

Vascular Endothelial Growth Factor 164 from rat

Name: Vascular Endothelial Growth Factor 164 from rat
Brand: SIGMA

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Synonym(s):

VEGF 164, VEGF-164, VEGF₁₆₄, Vasculotropin

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.32

MDL number:

MFCD01636149

Product Name

Vascular Endothelial Growth Factor 164 from rat, >95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

biological source

rat

recombinant

expressed in NSO cells

assay

>95% (SDS-PAGE)

form

lyophilized powder

potency

1.08 ng/mL ED₅₀

mol wt

dimer 50 kDa by SDS-PAGE
monomer 25 kDa by SDS-PAGE

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

UniProt accession no.

P16612

storage temp.

−20°C

Quality Level

200

Gene Information

rat ... Vegfa(83785)

Analysis Note

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in human umbilical vein endothelial cells.

Application

Vascular Endothelial Growth Factor 164 (VEGF 164) from rat was used for cardiomyocytes culture and Ca²⁺ transients′ measurement. It was used as a medium M-199 supplement utilized in in vitro incubation for examining the capability of CTS to limit the impact of biologically relevant molecules, in particular epidermal growth factor (EGF), basic fibroblast growth factor (FGF2), and vascular endothelial growth factor A (VEGFA) in rat epididymis segment 1 and 2.

Biochem/physiol Actions

Mitogen for endothelial cells, enhances vascular permeability, activates, and chemoattracts monocytes and is a procoagulant.

Vascular Endothelial Growth Factor 164 also referred to as VEGF 164 or Vasculotropin is a growth factor that induces endothelial cell growth, vasculogenesis and angiogenesis. VEGF 164 can enhance collagen and fibronectin expression in mesangial cells which in turn stimulates TGF-β1-induced extracellular matrix. It also plays a pivotal role in progressive glomerular fibrosis.

Other Notes

164 amino acid variant of rat VEGF.

Physical form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% TFA containing 0.5 mg bovine serum albumin.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Treatment with vascular endothelial growth factor-A worsens cognitive recovery in a rat model of mild traumatic brain injury.

Mujun Sun et al.

Frontiers in molecular neuroscience, 15, 937350-937350 (2022-11-18)

Mild traumatic brain injury (mTBI) is a common and unmet clinical issue, with limited treatments available to improve recovery. The cerebrovascular system is vital to provide oxygen and nutrition to the brain, and a growing body of research indicates that

Growth factor-stimulated mitogen-activated kinase (MAPK) phosphorylation in the rat epididymis is limited by segmental boundaries.

Jose L Tomsig et al.

Biology of reproduction, 75(4), 598-604 (2006-07-21)

Previous evidence has shown that sperm maturation is the result of successive events that influence sperm cells as they move through different microenvironments from the caput to the cauda epididymis. The physiological basis for the creation and maintenance of specific

VEGF-PLCgamma1 pathway controls cardiac contractility in the embryonic heart.

Wolfgang Rottbauer et al.

Genes & development, 19(13), 1624-1634 (2005-07-07)

The strength of the heart beat can accommodate in seconds to changes in blood pressure or flow. The mechanism for such homeostatic adaptation is unknown. We sought the cause of poor contractility in the heart of the embryonic zebrafish with

Transforming growth factor-beta1 stimulates vascular endothelial growth factor 164 via mitogen-activated protein kinase kinase 3-p38alpha and p38delta mitogen-activated protein kinase-dependent pathway in murine mesangial cells.

Lin Wang et al.

The Journal of biological chemistry, 279(32), 33213-33219 (2004-05-15)

Transforming growth factor-beta1 (TGF-beta1) is a potent inducer of extracellular matrix synthesis leading to progressive glomerular fibrosis. The intracellular signaling mechanisms involved in this process remain incompletely understood. The p38 mitogen-activated protein kinase (MAPK) is a major stress signal transducing

The splice variants of vascular endothelial growth factor (VEGF) and their receptors.

C J Robinson et al.

Journal of cell science, 114(Pt 5), 853-865 (2001-02-22)

Vascular endothelial growth factor (VEGF) is a secreted mitogen highly specific for cultured endothelial cells. In vivo VEGF induces microvascular permeability and plays a central role in both angiogenesis and vasculogenesis. VEGF is a promising target for therapeutic intervention in

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