Y2146

Yeast Synthetic Drop-out Medium Supplements

Name: Yeast Synthetic Drop-out Medium Supplements
Brand: SIGMA

without histidine, leucine, and tryptophan

Synonym(s):

Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements, Yeast media, amino acid dropout supplements

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All Photos(1)

About This Item

UNSPSC Code:

41106212

NACRES:

NA.85

MDL number:

MFCD01095679

Product Name

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, and tryptophan

form

powder

technique(s)

transformation: suitable

application(s)

microbiology

storage temp.

room temp

Quality Level

200

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Related Categories

Cloning & Expression

Industrial Microbiology

Microbial Culture Media

Molecular Cloning & Protein Expression

Application

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Yeast Synthetic Drop-out Medium Supplements are suitable for screening yeast transformants.

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Preparation Note

Dissolve 1.46 g of supplement per liter of medium.

pictograms

GHS05

signalword

Danger

hcodes

H318

pcodes

P280 - P305 + P351 + P338

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000410712

0000342960

0000206149

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Rab4A organizes endosomal domains for sorting cargo to lysosome-related organelles.

Sudeshna Nag et al.

Journal of cell science, 131(18) (2018-08-30)

Sorting endosomes (SEs) are the regulatory hubs for sorting cargo to multiple organelles, including lysosome-related organelles, such as melanosomes in melanocytes. In parallel, melanosome biogenesis is initiated from SEs with the processing and sequential transport of melanocyte-specific proteins toward maturing

STX13 regulates cargo delivery from recycling endosomes during melanosome biogenesis.

Riddhi Atul Jani et al.

Journal of cell science, 128(17), 3263-3276 (2015-07-26)

Melanosomes are a class of lysosome-related organelles produced by melanocytes. Biogenesis of melanosomes requires the transport of melanin-synthesizing enzymes from tubular recycling endosomes to maturing melanosomes. The SNARE proteins involved in these transport or fusion steps have been poorly studied.

Protocols

Yeast Drop Out

Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.