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Merck

577324-U

Ascentis® Express 90 Å AQ-C18 (2.7 μm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

Synonym(s):

Ascentis® Express 90 Å AQ-C18, 2.7 μm HPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
Particle size:
2.7 μm
Matrix active group:
AQ-C18, polar modified bonding phase
Pore size:
90 Å pore size
Matrix:
Fused-Core® silica particle platform, superficially porous particle

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Product Name

Ascentis® Express 90 Å AQ-C18, 2.7 μm HPLC Column, column L × I.D. 10 cm × 2.1 mm

material

stainless steel column

agency

suitable for USP L1

product line

Ascentis®

feature

endcapped

packaging

1 ea of

extent of labeling

6.5% Carbon loading

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

column L × I.D.

10 cm × 2.1 mm

surface area

135 m2/g

matrix

Fused-Core® silica particle platform, superficially porous particle

matrix active group

AQ-C18, polar modified bonding phase

particle size

2.7 μm

pore size

90 Å pore size

operating pH range

2-9

separation technique

reversed phase

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General description

Ascentis® Express AQ-C18 is a high-speed, high-performance liquid chromatography column based on the Fused-Core® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 μm thick porous shell and the small overall particle size of 2.7μm. The AQ-C18 is a C18 bonded phase prepared using a proprietary procedure that incorporates a small amount of polar silane which makes the phase resistant to dewetting. This resistance to dewetting allows the users of the AQ-C18 phase to run highly-aqueous (up to 100%) mobile phases. The modified C18 phase exhibits similar retention to Ascentis® Express C18 with a different selectivity, adding a valuable alternative for resolving difficult separations. The AQ-C18 is a reversed-phase packing that can be used for basic, acidic, and neutral compounds.

Application

UHPLC Analysis of Water Soluble Vitamins on Ascentis® Express AQ-C18, 2.7 μm
HPLC Analysis of Polar Compounds on Ascentis® Express C18 and AQ-C18, 2.7 μm
U/HPLC Analysis of Pyrethrins on Ascentis® Express AQ-C18, 2.7 μm

Analysis Note

The target analytes include acids, bases, and polar analytes.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Certificates of Analysis (COA)

Lot/Batch Number
USEAQ001286
USEAQ001285
USEAQ001284
USEAQ001283
USEAQ001282

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Liquid chromatography-mass spectrometry metabolite library for metabolomics: Evaluating column suitability using a scoring approach
Diamantidou D, et al.
Journal of Chromatography A, 1690, 463779-463779 (2023)
Dimitra Diamantidou et al.
Journal of chromatography. A, 1690, 463779-463779 (2023-01-22)
Untargeted metabolomic studies require an extensive set of analyte (metabolic) information to be obtained from each analyzed sample. Thus, highly selective, and efficient analytical methodologies together with reversed-phase (RP) or hydrophilic interaction liquid chromatography (HILIC) are usually applied in these

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