Within chromatin, the accessible regions of histones represent substrates for chromatin writer and eraser enzymes, as well as recognition domains for chromatin reader proteins that facilitate higher order structure important in chromatin, gene and nuclear regulation.
The AbSurance™ Pro Histone Peptide Microarray is a glass microarray containing peptides corresponding to sequences of all four core histone subunits (H2A, H2B, H3, H4) plus select variants. Over 260 histone peptides representing various post-translational modifications are present on the array as unmodified, singly modified as well as multiply modified sequences with up to six modifications per peptide. Each Microarray is printed as two subarrays (see figure 2) with each peptide represented six times per subarray to ensure confident binding analysis and quantitation.
Interaction analysis of histone readers, writers and erasers
The AbSurance™ Histone Antibody Specificity Arrays are essential screening tools to evaluate the specificity and cross-reactivity of antibodies against histones H2A, H2B, H3, H4, and their post-translational modifications. Two arrays provide a total of 89 unique high quality peptides in 10 ng and 100 ng quantities to easily detect both strong and weak cross-reactivity. The H3 Array provides 8 acetylated, 27 methylated, 5 phosphorylated, and 6 unmodified peptides of histone H3. The H2A, H2B, H4 Array offers 13 acetylated, 16 methylated, 6 phosphorylated, and 8 unmodified peptides of H2 and H4. Additional rat, sheep, rabbit, and mouse primary antibodies are spotted on each array for convenient built-in positive controls.