Sorting live cells based on intracellular RNA markers, followed by functional assays using the sorted cells, provides a unique opportunity to connect gene expression with cell function in heterogeneous cell populations, such as in tumors and immune cells.
Traditional live cell sorting, using antibodies against cell surface proteins or transfected reporter constructs, has several disadvantages, including poor cell recovery, compromised cell integrity and perturbed cellular pathways. The ability to sort live cells based on RNA expression overcomes this limitation and unlocks the potential to sort populations based on RNA biomarkers.
In this webcast, we describe a recently published method for sorting cells based on expression of specific RNA markers, without any transfection reagents or intrusive sample preparation1. You’ll learn how to perform RNA-based, live sorting of multiple cell types, including epithelial cells, mesenchymal cells, monocytes and macrophages. We will then show that the sorted cells retain their native biological functions by demonstrating their use in post-sort migration and bacterial phagocytosis assays.