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ST1549 Anti-FASN Mouse mAb (3F2-1F3)

Anti-FASN, mouse monoclonal, clone 3F2-1F3, recognizes FASN protein in 293 and HeLa cells and in human breast carcinoma tissue. It is validated for ELISA, WB, ICC, and IHC (paraffin sections).

Anti-FASN Mouse mAb (3F2-1F3): MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.

Sinonimi: Anti-Fatty Acid Synthase

ST1549
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
ST1549-50UG 50 μg
Description
OverviewRecognizes the FASN protein in 293 and HeLa cells and human breast carcinoma tissue.
Catalogue NumberST1549
Brand Family Calbiochem®
SynonymsAnti-Fatty Acid Synthase
Application Data
Detection of human FASN by immunoblotting. Sample: Whole cell lysate from 293 cells. Primary antibody: Anti-FASN Mouse mAb (3F2-1F3) (Cat. No. ST1549) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human FASN by staining paraffin sections. Sample: Formalin-fixed paraffin-embedded human breast cancer tissue. Primary antibody: Anti-FASN Mouse mAb (3F2-1F3) (Cat. No. ST1549) (10 µg/ml). Detection: colorimetric (DAB).

Detection of human FASN by immuncytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-FASN Mouse mAb (3F2-1F3) (Cat. No. ST1549) (10 µg/ml). Detection: fluorescence.
References
ReferencesKristiansen, G., et al. 2010. Br. J. Cancer 102, 1736.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive control293 cells, HeLa cells, Human breast carcinoma tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant human FASN (aa 1-440) expressed as GST fusion protein
Clone3F2-1F3
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
ST1549-50UG 04055977224252

Documentation

Anti-FASN Mouse mAb (3F2-1F3) MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-FASN Mouse mAb (3F2-1F3) Certificati d'Analisi

TitoloNumero di lotto
ST1549

Riferimenti bibliografici

Panoramica delle referenze
Kristiansen, G., et al. 2010. Br. J. Cancer 102, 1736.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-May-2010 RFH
SynonymsAnti-Fatty Acid Synthase
ApplicationELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (10 µg/ml)
Application Data
Detection of human FASN by immunoblotting. Sample: Whole cell lysate from 293 cells. Primary antibody: Anti-FASN Mouse mAb (3F2-1F3) (Cat. No. ST1549) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human FASN by staining paraffin sections. Sample: Formalin-fixed paraffin-embedded human breast cancer tissue. Primary antibody: Anti-FASN Mouse mAb (3F2-1F3) (Cat. No. ST1549) (10 µg/ml). Detection: colorimetric (DAB).

Detection of human FASN by immuncytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-FASN Mouse mAb (3F2-1F3) (Cat. No. ST1549) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the FASN protein.
BackgroundFASN is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-α), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-α.
HostMouse
ImmunogenFull-length, recombinant human FASN (aa 1-440) expressed as GST fusion protein
Clone3F2-1F3
IsotypeIgG1
Specieshuman
Positive control293 cells, HeLa cells, Human breast carcinoma tissue
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesKristiansen, G., et al. 2010. Br. J. Cancer 102, 1736.