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344145 FPTase, Rat, Recombinant, E. coli

344145
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Panoramica

Replacement Information

Tabella delle specifiche principali

Products

Numero di catalogoConfezionamento Qtà/conf
344145-100UG Fiala di plastica 100 μg
Description
OverviewRecombinant, rat FPTase expressed in E. coli. A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a variety of cellular proteins including Ras. Recognizes the C-terminal CAAX cysteine motif where the farnesylated cysteine is followed by two aliphatic amino acids and either leucine or serine.
M.W. α-subunit: ~48,000; β-subunit: ~46,000
Catalogue Number344145
Brand Family Calbiochem®
SynonymsFarnesyl Proteintransferase, FTase
References
ReferencesHightower, K.E., et al. 2001. Biochem. J. 360, 625.
Zimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
Reiss, Y., et al. 1990. Cell 62, 81.
Product Information
FormLiquid
FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Quality LevelMQ200
Applications
ApplicationFPTase, Rat, Recombinant, E. coli, a heterodimeric enzyme catalyzes the transfer of a 15-carbon isoprenoid group to a variety of cellular proteins. Recognizes the C-terminal CAAX cysteine motif.
Biological Information
Biological activity1 pmol of FPTase will transfer 1 pmol of farnesyl to H-Ras in 15 min at 37°C.
Purity≥85% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

FPTase, Rat, Recombinant, E. coli MSDS

Titolo

Scheda di sicurezza (MSDS) 

FPTase, Rat, Recombinant, E. coli Certificati d'Analisi

TitoloNumero di lotto
344145

Riferimenti bibliografici

Panoramica delle referenze
Hightower, K.E., et al. 2001. Biochem. J. 360, 625.
Zimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
Reiss, Y., et al. 1990. Cell 62, 81.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-October-2010 JSW
SynonymsFarnesyl Proteintransferase, FTase
DescriptionRecombinant, rat FPTase expressed in E. coli. A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a C-terminal cysteine in a variety of cellular proteins, including Ras. Recognizes the CaaX motif where the farnesylated cysteine (C) is followed by two aliphatic amino acids (aa) and either leucine or serine (X). Expressed in E. coli as an untagged recombinant protein.
FormLiquid
FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Purity≥85% by SDS-PAGE
Biological activity1 pmol of FPTase will transfer 1 pmol of farnesyl to H-Ras in 15 min at 37°C.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesHightower, K.E., et al. 2001. Biochem. J. 360, 625.
Zimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
Reiss, Y., et al. 1990. Cell 62, 81.