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422426 KGF/FGF-7, Mouse, Recombinant

422426
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Purity
≥90% by SDS-PAGE

Products

Numero di catalogoConfezionamento Qtà/conf
422426-25UG Bottiglia di vetro 25 μg
Description
OverviewRecombinant, mouse keratinocyte growth factor (KGF) consisting of amino acids 32-194 and expressed in E. coli. KGF plays an essential role in development, tumorigenesis, angiogenesis, morphogenesis, and wound healing. Mature mouse KGF amino acid sequence is 96% identical with rat, bovine, ovine, equine, and human forms and is active across species.
Catalogue Number422426
Brand Family Calbiochem®
SynonymsFGF-7
References
ReferencesBelleudi, F., et al. 2007. Traffic 8, 1854.
Finch, P.W. and J.S. Rubin. 2006. J. Natl. Cancer Inst. 98, 812.
de Georgi, V., et al. 2007. Dermatol. Clin. 25, 477.
Niu, J., et al. 2007. J. Biol. Chem. 282, 6001.
Werner, S., et al. 2007. J. Invest. Dermatol. 127, 998.
Cardinali, G., et al. 2005. J. Invest. Dermatol. 125, 1190.
Geer, D.J., et al. 2005. Am. J. Pathol. 167, 1575.
Hsu, YR., et al. 1999. Biochemistry 38, 2523.
Qiao, J., et al. 1999. Development 126, 547.
Werner, S., 1998. Cytokine Growth Factor Rev. 9, 153.
Guo, L., et al. 1996. Genes Dev. 10, 165.
Mason, I.J., et al. 1994. Mech. Dev. 45, 15.
Product Information
FormSolid
FormulationLyophilized from 0.2 µm sterile-filtered MOPS, Na₂SO₄, EDTA, DTT BSA.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 10-50 ng/ml as measured in a cell proliferation assay using 4MBr-5 monkey epithelial cells.
Purity≥90% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

KGF/FGF-7, Mouse, Recombinant MSDS

Titolo

Scheda di sicurezza (MSDS) 

KGF/FGF-7, Mouse, Recombinant Certificati d'Analisi

TitoloNumero di lotto
422426

Riferimenti bibliografici

Panoramica delle referenze
Belleudi, F., et al. 2007. Traffic 8, 1854.
Finch, P.W. and J.S. Rubin. 2006. J. Natl. Cancer Inst. 98, 812.
de Georgi, V., et al. 2007. Dermatol. Clin. 25, 477.
Niu, J., et al. 2007. J. Biol. Chem. 282, 6001.
Werner, S., et al. 2007. J. Invest. Dermatol. 127, 998.
Cardinali, G., et al. 2005. J. Invest. Dermatol. 125, 1190.
Geer, D.J., et al. 2005. Am. J. Pathol. 167, 1575.
Hsu, YR., et al. 1999. Biochemistry 38, 2523.
Qiao, J., et al. 1999. Development 126, 547.
Werner, S., 1998. Cytokine Growth Factor Rev. 9, 153.
Guo, L., et al. 1996. Genes Dev. 10, 165.
Mason, I.J., et al. 1994. Mech. Dev. 45, 15.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-Sepmtember-2011 RFH
SynonymsFGF-7
DescriptionRecombinant, mouse keratinocyte growth factor (KGF) consisting of amino acids 32-194 and expressed in E. coli. KGF plays an essential role in development, tumorigenesis, angiogenesis, morphogenesis, and wound healing. Mature mouse KGF amino acid sequence is 96% identical with rat, bovine, ovine, equine, and human forms and is active across species.
FormSolid
FormulationLyophilized from 0.2 µm sterile-filtered MOPS, Na₂SO₄, EDTA, DTT BSA.
Purity≥90% by SDS-PAGE
ContaminantsEndotoxin: <1.0 EU/µg protein
Biological activityED₅₀ = 10-50 ng/ml as measured in a cell proliferation assay using 4MBr-5 monkey epithelial cells.
SolubilityReconstitute to 250 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesBelleudi, F., et al. 2007. Traffic 8, 1854.
Finch, P.W. and J.S. Rubin. 2006. J. Natl. Cancer Inst. 98, 812.
de Georgi, V., et al. 2007. Dermatol. Clin. 25, 477.
Niu, J., et al. 2007. J. Biol. Chem. 282, 6001.
Werner, S., et al. 2007. J. Invest. Dermatol. 127, 998.
Cardinali, G., et al. 2005. J. Invest. Dermatol. 125, 1190.
Geer, D.J., et al. 2005. Am. J. Pathol. 167, 1575.
Hsu, YR., et al. 1999. Biochemistry 38, 2523.
Qiao, J., et al. 1999. Development 126, 547.
Werner, S., 1998. Cytokine Growth Factor Rev. 9, 153.
Guo, L., et al. 1996. Genes Dev. 10, 165.
Mason, I.J., et al. 1994. Mech. Dev. 45, 15.