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MF-Millipore™ Membrane Filters

Mixed cellulose esters

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Specifiche tecniche

Informazioni per l’ordine

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Standard MF-Millipore Membranes, white, plain · Catalogue No.Azzera le impostazioni per l'ordine ed i filtri Show Filter
Numero di catalogoDimensioni dei poriDiametro del filtro (⌀)Q.tà/ conf.
VSWP013000.025 µm 13 mm 100
VSWP025000.025 µm 25 mm 100
VSWP047000.025 µm 47 mm 100
VSWP090250.025 µm 90 mm 25
VSWP142500.025 µm 142 mm 50
VSWP293250.025 µm 293 mm 25
VMWP013000.05 µm 13 mm 100
VMWP025000.05 µm 25 mm 100
VMWP047000.05 µm 47 mm 100
VMWP090250.05 µm 90 mm 25
VCWP013000.1 µm 13 mm 100
VCWP025000.1 µm 25 mm 100
VCWP047000.1 µm 47 mm 100
VCWP090250.1 µm 90 mm 25
VCWP142500.1 µm 142 mm 50
VCWP293250.1 µm 293 mm 25
GSWP013000.22 µm 13 mm 100
GSWP025000.22 µm 25 mm 100
GSWP047000.22 µm 47 mm 100
GSWP037000.22 µm 37 mm 100
GSWP090000.22 µm 90 mm 100
GSWP142500.22 µm 142 mm 50
GSWP293250.22 µm 293 mm 25
PHWP025000.3 µm 25 mm 100
PHWP047000.3 µm 47 mm 100
PHWP090250.3 µm 90 mm 25
PHWP142500.3 µm 142 mm 50
PHWP293250.3 µm 293 mm 25
HAWP013000.45 µm 13 mm 100
HAWP024000.45 µm 24 mm 100
HAWP037000.45 µm 37 mm 100
HAWP047000.45 µm 47 mm 100
HAWP04700F10.45 µm 47 mm Hydrophilic, 0.45µm, White Plain 47 mm, 100
HAWP0470M0.45 µm 47 mm 50 coppie
HAWP025000.45 µm 25 mm 100
HAWP050000.45 µm 50 mm 100
HAWP090000.45 µm 90 mm 100
HAWP142500.45 µm 142 mm 50
HAWP293250.45 µm 293 mm 25
DAWP013000.65 µm 13 mm 100
DAWP025000.65 µm 25 mm 100
DAWP047000.65 µm 47 mm 100
DAWP090250.65 µm 90 mm 25
DAWP142500.65 µm 142 mm 50
DAWP293250.65 µm 293 mm 25
AAWP013000.8 µm 13 mm 100
AAWP025000.8 µm 25 mm 100
AAWP037000.8 µm 37 mm 100
AAWP037PM0.8 µm 37 mm 50 coppie
AAWP037P00.8 µm 37 mm 100
AAWP047000.8 µm 47 mm 100
AAWP0470M0.8 µm 47 mm 50 coppie
AAWP090000.8 µm 90 mm 100
AAWP090500.8 µm 90 mm 50
AAWP142500.8 µm 142 mm 50
AAWP293250.8 µm 293 mm 25
RAWP013001.2 µm 13 mm 100
RAWP025001.2 µm 25 mm 100
RAWP037001.2 µm 37 mm 100
RAWP047001.2 µm 47 mm 100
RAWP090251.2 µm 90 mm 25
RAWP293251.2 µm 293 mm 25
RAWP142501.2 µm 142 mm 50
SSWP013003.0 µm 13 mm 100
SSWP047003.0 µm 47 mm 100
SSWP025003.0 µm 25 mm 100
SSWP090253.0 µm 90 mm 25
SSWP142503.0 µm 142 mm 50
SSWP293253.0 µm 293 mm 25
SMWP013005.0 µm 13 mm 100
SMWP0190R5.0 µm 不适用 100
SMWP025005.0 µm 25 mm 100
SMWP037005.0 µm 37 mm 100
SMWP047005.0 µm 47 mm 100
SMWP090255.0 µm 90 mm 25
SMWP142505.0 µm 142 mm 50
SMWP293255.0 µm 293 mm 25
SCWP013008.0 µm 13 mm 100
SCWP0190R8.0 µm 不适用 100
SCWP025008.0 µm 25 mm 100
SCWP047008.0 µm 47 mm 100
SCWP090258.0 µm 90 mm 25
SCWP142508.0 µm 142 mm 50
SCWP293258.0 µm 293 mm 25
HAWG047050.45 µm 47 mm 500

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Standard MF-Millipore Membranes, white, griddedAzzera le impostazioni per l'ordine ed i filtri Show Filter
Numero di catalogoDimensioni dei poriDiametro del filtro (⌀)Q.tà/ conf.
HAWG013000.45 µm 13 mm 100
HAWG025000.45 µm 25 mm 100
HAWG037000.45 µm 37 mm 100
HAWG047000.45 µm 47 mm 100
HAWG047050.45 µm 47 mm 500
AAWG013000.8 µm 13 mm 100
AAWG0250C0.8 µm 25 mm 100
AAWG037000.8 µm 37 mm 100
AAWG047000.8 µm 47 mm 100
RAWG025001.2 µm 25 mm 100
RAWG0250C1.2 µm 25 mm 100
RAWG047001.2 µm 47 mm 100

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Standard MF-Millipore Membranes, black, plainAzzera le impostazioni per l'ordine ed i filtri Show Filter
Numero di catalogoDimensioni dei poriDiametro del filtro (⌀)Q.tà/ conf.
HABP025000.45 µm 25 mm 100
HABP047000.45 µm 47 mm 100
AABP025000.8 µm 25 mm 100
AABP047000.8 µm 47 mm 100

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Standard MF-Millipore Membranes, black, griddedAzzera le impostazioni per l'ordine ed i filtri Show Filter
Numero di catalogoDimensioni dei poriDiametro del filtro (⌀)Q.tà/ conf.
AABG013000.8 µm 13 mm 100
AABG025000.8 µm 25 mm 100
AABG037000.8 µm 37 mm 100
AABG047000.8 µm 47 mm 100
HABG013000.45 µm 13 mm 100
HABG025000.45 µm 25 mm 100
HABG047000.45 µm 47 mm 100

(1)Monitor refills with thin absorbent pads for aerosol monitoring.
(2)Matched weight filter pairs.
(3)Monitor refills with thick absorbent pads for liquid monitoring.
(4)Monitor refills (matched weight pairs) with thick absorbent pads for liquid monitoring.
(5)Cut from specifically selected and controlled roll stock to avoid contamination by fibers. For asbestos monitoring applications.

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MF-Millipore Membranes without Triton surfactant, white, plainAzzera le impostazioni per l'ordine ed i filtri Show Filter
Numero di catalogoDimensioni dei poriDiametro del filtro (⌀)Q.tà/ conf.
GSTF013000.22 µm 13 mm 100
GSTF025000.22 µm 25 mm 100
GSTF047000.22 µm 47 mm 100
GSTF090000.22 µm 90 mm 100
GSTF142500.22 µm 142 mm 50
GSTF293250.22 µm 293 mm 25
HATF013000.45 µm 13 mm 100
HATF025000.45 µm 25 mm 100
HATF047000.45 µm 47 mm 100
HATF082500.45 µm 82 mm 50
HATF085500.45 µm 85 mm 50
HATF090250.45 µm 90 mm 25
HATF100500.45 µm 100 mm 50
HATF142500.45 µm 142 mm 50
HATF293250.45 µm 293 mm 25
RATF047001.2 µm 47 mm 100
RATF293251.2 µm 293 mm 25

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AccessoryAzzera le impostazioni per l'ordine ed i filtri Show Filter
Numero di catalogoDescrizioneQ.tà/ conf.
XX6200006PPinzette a punte piatte per filtri, acciaio inossidabile 3

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Documentation

Scheda tecnica

Titolo
Chemical Compatibility of Filter Components (Merck)
MF-Millipore Filters: A biologically inert mixture of cellulose acetate and cellulose nitrate

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiApplicazione
Mobile Phase Preparation for UHPLC: Membrane Filtration Method Affects System Performance and Leaching of Extractable Impurities
Subodh Kulkarn(1), Jesmi George(2) and Vivek Joshi(2) (1) Millipore India Pvt. Ltd., Bioscience Division, 50A, 2nd Phase, Ring Road, Peenya, Bangalore, India 560058 (2) Millipore Corp., Bioscience Division, 17 Cherry Hill Drive, Danvers, MA 01923
LCGC  2009

Mostra il sommario Testo completo dell'articolo
Direct Effect of Vanadium on Citrate Uptake by Rat Renal Brush Border Membrane Vesicles (BBMV)
Kazuhiro Sato, et al.
Industrial Health , 40, 278-281, 2002, internal ref BB02  2002

Effect of Platinum Coordination Complex (PtCx) on Citrate Uptake by Rat Renal Bush Border Membrane vesicles (BBMV):Direct Effect of Carboplatin
Kazuhiro Sato, et al.
Industrial Health, 39, 21-31, 2001; internal ref BB01  2001

A sensitive Elispot assay to detect low-frequency human T-lymphocytes
McCutcheon M, Wehner N, Wensky A et al.
J Immunol Methods 210(2): 149-166.  1997

ELISPOT Assays
Direct effect of cadmium on citrate uptake by isolated rat renal brush border membrane cesicles
Kazuhiro Sato, et al.
Toxicology Letters, 80, 161-165, 1995, internal ref BB06  1994

Stoichiometry of Na+-Succinate Cotransport in Renla Brush-Border Membranes
Stephen H. at al.,Journal of Biological Chemistry, 257, 4, 1773-1778, 1982, Internal ref bb03
Journal of Biological Chemistry, 257, 4, 1773-1778, 1982, Internal ref bb03  1981

FAQ

DomandaRisposta
What would you recommend to dissolve MF ( mixed cellulose ester ) filters?Many solvents are possible. A popular choice is acetone, which is non-toxic and highly volatile so it is easy to evaporate (but quite a flammable). Another choice is NMP (n-methylpyrrolidinone), which is not very volatile at room temperature. The selection is often based on what is to be done after the dissolution.
How do I clear my Millipore filters?It is possible to clear Millipore filters by choosing a clearing agent with the same refractive index as the membrane. This is called the PORE FILLING TECHNIQUE. Filters appear opaque because of the diffraction of light through the tortuous pores. Filling the pores with a liquid that has the same refractive index as the membrane (ex. xylene for MF filters) allows light to pass through the filter at a uniform speed thus rendering the filter transparent. This technique can be used for most membrane filters with a single refractive index. The refractive index for Durapore membrane is 1.42 and for MF membrane its 1.50.

Please note that this does not work for polycarbonate membranes because these membranes have more than one refractive index (1.62 and 1.58). If desired, the porous structure can be dissolved from Isopore polycarbonates by dissolving the filter in chloroform, methylene chloride or 1-methyl-2 pyrrolidone.
What is the blue separation paper between my filters?It is a parchmentalized paper specially formulated for Millipore Corporation.
How do I assemble a diffusion chamber containing tissue for implantation?
  1. Wet a single membrane filter (either GSWP 013 00 or HAWP 013 00) with Milli-Q water and blot it to absorbent paper to remove excess water.
  2. Glue the filter to one side of a diffusion chamber ring without hole (catalog number PR00 014 00), using MF cement (catalog number XX70 000 00), and let dry.
  3. Sterilize this assembly, along with another membrane filter of the same type, by exposure to ethylene oxide gas or ultraviolet light.
  4. Moisten the half-completed chamber and the separate sterile filter with a sterile physiological solution, and aseptically place the tissue to be studied in the half-completed chamber assembly.
  5. Glue the cover filter in place using sterile technique.
What are the dimensions and width of the grids on the Millipore 47mm gridded membranes?The grids are 3.08mm x 3.08mm square. A grid line is 44um in width and there is are average of 169 gridded squares per filter. Please note however that the actual filtration area and therefore the total number of squares in that area is dependent on the filter holder. For filter holders with 9.6sq.cm of filtration area there are 100 squares available. For filter holders with 13.8 sq.cm of filtration area the number of available grids are approximately 140.
How do I assemble a diffusion chamber containing suspension cells for implantation?Wet two membrane filters (either GSWP 013 00 or HAWP 013 00) with Milli-Q water and blot them to absorbent paper to remove excess water. Glue a moist filter to each side of the diffusion chamber ring with hole (catalog number PR00 014 01) using MF cement (catalog number XX70 000 00). Sterilize the dried diffusion chamber with ethylene oxide gas or ultraviolet light. After sterilization, inject the cells through the hole in the plastic ring, and plug the hole with the nylon thread which comes with the rings (catalog number PR00 000 00 if ordered separately).
Which membrane should I use to filter my solution?For general filtration use MCE. When you are looking for the lowest protein binding membrane use Durapore. For speed, and when filtering serum solutions use Millipore Express.
Which side of the membrane should I use, the shiny or dull side?Most researchers may not even notice that there is a "sidedness" to filters, and, for most applications, orientation will not affect filter performance. However, membranes do have a slightly asymmetric pore structure: the shiny side of the membrane is the "tighter" side. In some applications, you can take advantage of this difference by selecting a specific filter orientation. ultrafiltration membranes should always be used shiny side up, regardless of application for drop dialysis ( a buffer exchange technique in which a few drops of DNA or protein are placed in a 0.05 or 0.025 um filter and floated on a buffer solution), apply the sample to the shiny side of the filter and float the filter dull side to the buffer. This measure will enhance buffer exchange and discourage sample loss. The Millipore Express and Express Plus membranees are also sided - these membranes should be used shiny side facing down.
What filter do you recommend for dialyzing 5-100ul sample volumes of proteins and nucleic acids (i.e. to perform a drop dialysis technique)?With the Millipore drop dialysis technique 0.025 um or 0.05 um MF mixed cellulose ester membranes are commonly used. This simple- to-use technique takes advantage of the varying diffusion rates of different size molecules to quickly dialyze small volume samples. The technique involves filling the bottom of a Petri dish with dialysate and floating the membrane filter disc, shiny side up, on the surface of the solution. You then deposit 5-100 ul samples on the center of the disc with a micropipet. use of dye markers in the sample may help to determine the dialysis time required. Most samples are dialyzed in less than 30 minutes. then simply recover the desalted sample with a micropipet.
How do I tell the membrane from the separator?The blue paper is the separator.