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Merck

WBULS0500

Immobilon ECL Ultra Western HRP Substrate

Western blotting, HRP

Synonym(s):

chemiluminescent detection substrate, detection substrate

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About This Item

UNSPSC Code:
41116010
NACRES:
NA.32
eCl@ss:
42029053
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technique(s)

western blot: suitable

detection method

chemiluminescent

shipped in

ambient

Quality Level

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General description

Immobilon® ECL Ultra Western HRP Substrate is an ultra-sensitive reagent with long signal duration for the detection of horseradish peroxidase (HRP)-labeled antibodies on western blots. The intense signal created permits sensitive detection at the mid-to-low femtogram range using x-ray film or digital imaging systems.

Application

"Immobilon® ECL Ultra substrate is compatible with both PVDF andnitrocellulose blotting membranes, as well as commonly used buffers and blocking reagents.

Sufficient for 5000 cm²"
Western blotting, HRP

Preparation Note

Store at 2–8 °C

Other Notes

Solution A- 250 mL

Solution B- 250 mL

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Please refer to KIT Component information

pdsc

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prtr

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fsl

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ishl_indicated

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ishl_notified

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Protocols

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

ウェスタンブロッティングでは、ゲル電気泳動によるタンパク質の分離、ポリフッ化ビニリデン(PVDF)またはニトロセルロースメンブレンへの転写、さまざまな方法による検出が行われます。

Related Content

We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

我々はそれを理解している。ウエスタンはしみ抜きで苦労することがある。そこで、あなたの醜いブロットを私たちと共有し、サプライズを受け取ってください!それが何であるかは言えませんが-それは驚きを台無しにしてしまうからです-ラボの創造性を掻き立てるということを除いては。

Global Trade Item Number

SKUGTIN
WBULS050004054839180972

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