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Merck

E4382

Eosin Y disodium salt

Dye content, ≥90%, certified by the Biological Stain Commission, powder

Synonym(s):

2′,4′,5′,7′-Tetrabromofluorescein disodium salt, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, D&C;Red No. 22, Eosin yellowish

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About This Item

Empirical Formula (Hill Notation):
C20H6Br4Na2O5
CAS Number:
17372-87-1
Molecular Weight:
691.85
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24894551
EC Number:
241-409-6
MDL number:
MFCD00005040
Colour Index Number:
45380
Beilstein/REAXYS Number:
3586809

Key Documents

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Product Name

Eosin Y disodium salt, certified by the BSC

InChI key

SEACYXSIPDVVMV-UHFFFAOYSA-L

InChI

1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

SMILES string

[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24

agency

certified by the BSC

form

powder

composition

Dye content, ≥90%

technique(s)

titration: suitable

color

brown

pH

6.55 (29 °C, 84 °F)

mp

255 — 270 °C (491 - 518 °F at 977.9 hPa)

solubility

H2O: 1 mg/mL

density

1.018 g/cm3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Related Categories

Application

Certified for use as a counterstain against hematoxylin; as a constituent of Wright stain; in modified Nocht′s stain; as a constituent in eosin-methylene blue medium.
Eosin Y disodium salt has been used as a counterstain in immunocytochemistry and immunohistochemistry.

Biochem/physiol Actions

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317,H319

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

376.9 °F - Pensky-Martens closed cup

flash_point_c

191.6 °C - Pensky-Martens closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

E4382-BULK: + E4382-100G: + E4382-VAR: + E4382-25G: + E4382-H:

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Certificates of Analysis (COA)

Lot/Batch Number
0000525103
0000525103
0000525130
0000525130
0000510152

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Dendritic cell loss from nonlymphoid tissues after systemic administration of lipopolysaccharide, tumor necrosis factor, and interleukin 1.
Roake JA, et al.
The Journal of Experimental Medicine, 181, 2237-2237 (1995)
Different expression of occludin and ZO-1 in primary and metastatic liver tumors.
Orban E, et al.
Pathology Oncology Research, 14, 299-299 (2008)
Bancroft JD
Theory and Practice of Histological Techniques (2008)
Joanne M Donkers et al.
Cellular and molecular gastroenterology and hepatology, 10(3), 451-466 (2020-04-25)
Bile acids are important metabolic signaling molecules. Bile acid receptor activation promotes body weight loss and improves glycemic control. The incretin hormone GLP-1 and thyroid hormone activation of T4 to T3 have been suggested as important contributors. Here, we identify
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