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Merck

M8884

Methyl Green

~85% purity, powder

Synonym(s):

Ethyl Green

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About This Item

Linear Formula:
C27H35BrClN3 · ZnCl2
CAS Number:
7114-03-6
Molecular Weight:
653.24
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24897293
EC Number:
230-415-4
MDL number:
MFCD00151094
Colour Index Number:
42590
Beilstein/REAXYS Number:
3914106

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Product Name

Methyl Green, zinc chloride salt, ~85%

InChI

1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

InChI key

RRKSQWOQZFIQKW-UHFFFAOYSA-J

SMILES string

[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C

assay

~85%

form

powder

composition

Dye Content, ≥83%

technique(s)

titration: suitable

color

red to very dark red

pH

0.2-1.8, yellow to blue

mp

>300 °C (lit.)

solubility

water: 1 mg/mL

εmax

>11100.00 at 417-425 nm in water at 0.005 g/L, >16000 at 311-319 nm in water at 0.005 g/L, >60000 at 626-634 nm in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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General description

Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.

Application

Methyl green is suitable as a nuclear counterstain in enzyme histochemistry, immunostaining, and in situ hybridization as the bluish-green color will be in contrast with red, purple, brown, and black products of histochemical reactions. It is also suitable as a tracking dye in Acid-polyacrylamide gel electrophoresis to separate gliadins. It has been used in the histochemical staining for the apoptosis examination of kidney tissue and as a nuclear stain for labeling embryos.

Analysis Note

Dye content based on molecular weight of monobromo-monochloro ZnCl2 salt (653.2).
Material may be a mixture of monobromo-monochloro and dichloro ZnCl2 salts. Halogen contents listed on label.

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Class I Designated Chemical Substances

prtr

M8884-5G: + M8884-1G: + M8884-VAR: + M8884-25G: + M8884-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT0860
SHBS0900
SHBR4060
SHBP2836
SHBP8900

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