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Merck

75633

Osmium tetroxide solution

suitable for electron microscopy, 2% in H2O

Synonym(s):

Osmium(IV) oxide

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About This Item

Linear Formula:
OsO4
CAS Number:
20816-12-0
Molecular Weight:
254.23
NACRES:
NA.47
PubChem Substance ID:
57652577
UNSPSC Code:
12171500
MDL number:
MFCD00011150
Form:
solution

Key Documents

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InChI

1S/4O.Os

SMILES string

O=[Os](=O)(=O)=O

InChI key

VUVGYHUDAICLFK-UHFFFAOYSA-N

form

solution

reaction suitability

reagent type: oxidant

concentration

2% in H2O

technique(s)

electron microscopy: suitable

color

colorless to yellow

density

1.0 g/mL at 20 °C, 1.0 g/mL at 20 °C

application(s)

hematology
histology

storage temp.

2-8°C

Quality Level

100

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General description

Osmium tetroxide (OsO4) also referred to as osmium, is a heavy tetrahedral, symmetrical molecule. It is both a dye and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium tetroxide to ensure the preservation of the lipids.

Application

Osmium tetroxide solution has been used as a fixative in electron microscopy.

Biochem/physiol Actions

Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.

pictograms

Skull and crossbonesCorrosion
GHS06,GHS05

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Storage Class

6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

75633-VAR: + 75633-1EA-BULK: + 75633-1EA: + 75633-2ML:4548173353258 + 75633-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN1960
BCCL4006
BCCK2370
BCCH6755
BCCF5871

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