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Merck

C0528

Casein fluorescein isothiocyanate from bovine milk

Type III, essentially salt-free, lyophilized powder

Synonym(s):

FITC-casein

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
MFCD00130743

Key Documents

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type

Type III

form

essentially salt-free, lyophilized powder

extent of labeling

≥50 μg FITC per mg solid

solubility

water: 5 mg/mL, clear to hazy

storage temp.

2-8°C

Quality Level

200

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Application

Casein fluorescein isothiocyanate from bovine milk has been used:
  • in accessing zona pellucida (ZP) and perivitelline space (PVS) to evaluate changes induced by protease treatment
  • to make a reference line in immunochromatographic devices
  • in running gel for the documentation of proteinases

highly sensitive protease substrate

Biochem/physiol Actions

Casein fluorescein isothiocyanate is mainly used as a substrate in estimating the protease activity of psychrotrophic bacteria. It is also used as a substrate for determining the activity of bacteria in milk.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

C0528-25MG: + C0528-100MG: + C0528-10MG: + C0528-BULK: + C0528-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
0000530552
0000530553
0000530552
0000530553
0000515246

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The supplementation of culture medium with protease improves the hatching rate of mouse embryos
Lee DR, et al.
Human Reproduction, 12(11), 2493-2498 (1997)
Characteristics of digestive enzymes of calanoid copepod species from different latitudes in relation to temperature, pH and food
Freese D, et al.
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 162(4), 66-72 (2012)
Immunochromatographic assay for simple and rapid detection of Satsuma dwarf virus and related viruses using monoclonal antibodies
Kusano N, et al.
Journal of General Plant Pathology, 73(1), 66-71 (2007)
Casein, fluorescein isothiocyanate as a substrate for measuring protease production by psychrotrophic bacteria
Christen GL and Senica M
Journal of Food Protection, 50(9), 744-749 (1987)
Brent Cezairliyan et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(37), E7796-E7802 (2017-08-30)
Pathogenic bacteria secrete toxins and degradative enzymes that facilitate their growth by liberating nutrients from the environment. To understand bacterial growth under nutrient-limited conditions, we studied resource allocation between cellular and secreted components by the pathogenic bacterium
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