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Merck

C5041

6-Carboxyfluorescein diacetate

~95% purity (HPLC), powder

Synonym(s):

6-CFDA, 6-Carboxy-di-O-acetylfluorescein

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About This Item

Empirical Formula (Hill Notation):
C25H16O9
CAS Number:
3348-03-6
Molecular Weight:
460.39
NACRES:
NA.47
PubChem Substance ID:
24892748
UNSPSC Code:
12171500
MDL number:
MFCD00036871
Beilstein/REAXYS Number:
67043

Key Documents

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Product Name

6-Carboxyfluorescein diacetate, ≥95% (HPLC)

InChI

1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1

InChI key

QMOGCCYGOPYYNT-UHFFFAOYSA-N

assay

≥95% (HPLC)

form

powder

color

white to yellow

solubility

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

Quality Level

100, 200

Related Categories

Application

6-Carboxyfluorescein diacetate has been used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin.
Fluorescent wavelengths λex 492nm; λemm 517nm in 0.1M Tris, pH 8.0.

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

C5041-100MG: + C5041-VAR: + C5041-10UG-PW: + C5041-10UG: + C5041-25MG-PW: + C5041-BULK: + C5041-100MG-PW: + C5041-25MG: + C5041-10MG:

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Certificates of Analysis (COA)

Lot/Batch Number
0000497414
0000497414
0000379921
0000350050
0000329914

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G Ricci et al.
Human reproduction (Oxford, England), 17(10), 2665-2672 (2002-09-28)
Apoptosis plays an important role in regulating spermatogenesis. However, the biological significance of apoptosis in ejaculated sperm is not yet clear. This study set out to investigate how apoptosis correlates with semen quality and the presence of seminal leukocytes. Fifty-seven
Garry R Smith et al.
Journal of molecular neuroscience : MN, 52(3), 446-458 (2013-11-28)
Severe seizure activity is associated with reoccurring cycles of excitotoxicity and oxidative stress that result in progressive neuronal damage and death. Intervention with these pathological processes is a compelling disease-modifying strategy for the treatment of seizure disorders. We have optimized
Evaluation of sperm quality snakes Erythrolamprus poecilogyrus sublineatus (Cope, 1860) (Serpentes, Dipsadidae).
Silva AC, et al.
Brazilian journal of biology = Revista brasleira de biologia, doi: 10-doi: 10 (2017)
Gopal Patel et al.
Frontiers in bioengineering and biotechnology, 8, 631-631 (2020-07-03)
The present study explores the influence of mycophenolic acid (MPA) in combination therapy with quercetin (QC) (impeding MPA metabolic rate) delivered using the liposomal nanoparticles (LNPs). Mycophenolic acid liposome nanoparticles (MPA-LNPs) and quercetin liposome nanoparticles (QC-LNPs) were individually prepared and
S Capellero et al.
Scientific reports, 10(1), 6478-6478 (2020-04-17)
Despite improvements in surgery and medical treatments, epithelial ovarian cancer (EOC) remains the most lethal gynaecological malignancy. Aim of this study is to investigate the preclinical immunotherapy activity of cytokine-induced killer lymphocytes (CIK) against epithelial ovarian cancers, focusing on platinum-resistant
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