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Merck

C6144

Chlorophyll a

Powder

Synonym(s):

Pheophytin; universal photosynthetic pigment

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About This Item

Empirical Formula (Hill Notation):
C55H72MgN4O5
CAS Number:
479-61-8
Molecular Weight:
893.49
NACRES:
NA.47
PubChem Substance ID:
24892839
UNSPSC Code:
12171500
EC Number:
207-536-6
MDL number:
MFCD00079050
Beilstein/REAXYS Number:
3586237

Key Documents

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Product Name

Chlorophyll a, from Anacystis nidulans algae

InChI

1S/C55H73N4O5.Mg/c1-13-39-35(8)42-28-44-37(10)41(24-25-48(60)64-27-26-34(7)23-17-22-33(6)21-16-20-32(5)19-15-18-31(3)4)52(58-44)50-51(55(62)63-12)54(61)49-38(11)45(59-53(49)50)30-47-40(14-2)36(9)43(57-47)29-46(39)56-42;/h13,26,28-33,37,41,51H,1,14-25,27H2,2-12H3,(H-,56,57,58,59,61);/q-1;+2/p-1/b34-26+;/t32-,33-,37+,41+,51-;/m1./s1

InChI key

ATNHDLDRLWWWCB-AENOIHSZSA-M

SMILES string

CCc1c(C)c2cc3c(C=C)c(C)c4cc5nc([C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@@H]5C)c6[C@@H](C(=O)OC)C(=O)c7c(C)c(cc1n2)n([Mg]n34)c67

biological source

Anacystis nidulans algae

form

powder

technique(s)

titration: suitable

impurities

chlorophyll b, free

color

blue to very dark blue

mp

150-153 °C

solubility

diethyl ether: 0.1 mg/10 mL

ε (extinction coefficient)

11.1-12.1 × 104 at 428-430 nm in diethyl ether, 8.3-9.4 × 104 at 660-662 nm in diethyl ether

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

Quality Level

200

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Application

A procedure has been described for harvesting native light-harvesting complex IIb (LHCIIb).
Chlorophyll a has been used as a standard for the calibration of fluorometer.

Biochem/physiol Actions

Chlorophyll a is an essential pigment in oxygenic photosynthesis. In photosystems, it is present as an antenna pigment as well as in reaction center I and II. Chlorophyll a is a blue-green pigment which absorbs light in 400-450nm and 640-660nm range. In photosynthesis, the energy absorbed by chlorophyll transforms carbon dioxide and water into carbohydrates and oxygen. When chlorophyll absorbs light energy, an electron in chlorophyll is excited from a lower energy state to a higher energy state. In this higher energy state, the electron is more readily transferred to another molecule. This starts a chain of electron-transfer steps, which ends with an electron transferred to carbon dioxide.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.
Sealed ampule.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

C6144-10MG: + C6144-5MG: + C6144-BULK: + C6144-VAR: + C6144-1MG:

jan

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN8783
BCCN3411
BCCL5930
BCCM5739
BCCK5088

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Li H, et al.
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