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HT501128

Formalin solution, neutral buffered, 10%

Name: Formalin solution, neutral buffered, 10%
Brand: SIGMA

histological tissue fixative

Synonym(s):

Formaldehyde, Methanal

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About This Item

UNSPSC Code:

12352114

PubChem Substance ID:

24895887

NACRES:

NA.47

MDL number:

MFCD00003274

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Properties

biological source

synthetic

Quality Level

200

concentration

10% formalin (approx. 4% formaldehyde)

color

colorless

pH

6.90-7.10

density

1.080 g/cm³

application(s)

hematology
histology

storage temp.

room temp

SMILES string

[O-]#[C+H2]

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

Description

General description

10% Neutral buffered formalin (NBF) is the most commonly used fixative throughout the world for light microscopy and is a somewhat forgiving fixative. 10% NBF is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.

Application

Formalin solution, neutral buffered, 10% has been used in oil-red-o staining for the differentiation of 3T3-L1 preadipocyte cell line and mouse embryonic fibroblasts into adipocytes. It has been used in the histological analysis of rabbit′s eye and diabetic rat intestine.

Formalin solution, neutral buffered, 10% has been used as a popular general purpose histological fixative to fix tissues.

Popular general purpose histological fixative.

Physical form

Neutral buffered solution

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H302 + H332,H317,H341,H350

pcodes

P202 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P308 + P313

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

wgk

WGK 3

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Deleterious substance

pdsc

Specified Class I Designated Chemical Substances

prtr

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

HT501128-VAR: + HT501128-BULK: + HT501128-4L:4548173199825 + HT501128-4L-H: + HT501128-4L-PW:

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