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Merck

I1284

Isopropyl β-D-thiogalactopyranoside solution

ReadyMade IPTG solution for Blue-white screening

Synonym(s):

IPTG solution

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About This Item

Empirical Formula (Hill Notation):
C9H18O5S
CAS Number:
367-93-1
Molecular Weight:
238.30
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
24895928
MDL number:
MFCD00063273
Beilstein/REAXYS Number:
4631
Form:
liquid
Quality level:
200

Key Documents

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Product Name

Isopropyl β-D-thiogalactopyranoside solution, ReadyMade IPTG solution for Blue-white screening

Quality Level

200

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

SMILES string

CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O

InChI key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

grade

Molecular Biology

description

non-ionic

form

liquid

mol wt

238.3

shipped in

dry ice

storage temp.

−20°C

Related Categories

Application

Isopropyl β-D-thiogalactopyranoside solution is:
  • used as an inducer for the expression of single domain antibodies(sdAbs) in E coli and bioactive peptides
  • used in its half-life and decay studies
  • suitable for induction of recombinant protein expression in systems controlled by the lac repressor

Biochem/physiol Actions

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized by E. coli its concentration remains constant. Therefore, it is not a variable in the experiment.
Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product.

General description

Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 °C), thus avoiding freeze-thaw cycles and providing a user-friendly solution.

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

I1284-5ML: + I1284-5ML-PW: + I1284-BULK: + I1284-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
0000505949
0000505949
0000439782
0000439782
0000398760

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Engineering of ultra-small diagnostic nanoprobes through oriented conjugation of single-domain antibodies and quantum dots
Sukhanova A, et al.
Protocol Exchange, 10 (2012)
Half-life measurements of chemical inducers for recombinant gene expression
Pasotti L, et al.
Journal of Biological Engineering, 8(1), 5-5 (2014)
Random sequences are an abundant source of bioactive RNAs or peptides
Neme R, et al.
Nature ecology & evolution, 1(6), 0127-0127 (2017)
L H Hansen et al.
Current microbiology, 36(6), 341-347 (1998-06-03)
The role of the Escherichia coli lacY gene product (the lactose permease) in the induction of isopropyl-beta-D-thiogalactopyranoside (IPTG) inducible promoters was studied in E. coli and P. fluorescens. This was done by comparing strains containing a lacIPOZYA chromosomal insert with
Iltaf Ahmed et al.
Human molecular genetics, 24(11), 3172-3180 (2015-02-24)
There are two known mRNA degradation pathways, 3' to 5' and 5' to 3'. We identified likely pathogenic variants in two genes involved in these two pathways in individuals with intellectual disability. In a large family with multiple branches, we
View All Related Papers

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