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Merck

L7025

LB Broth with agar (Lennox)

Tablet microbial growth medium

Synonym(s):

LB Agar, LB agar

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About This Item

NACRES:
NA.85
UNSPSC Code:
12352200

Key Documents

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grade

Molecular Biology

sterility

non-sterile

form

tablet

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

Quality Level

200

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General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Features and Benefits

Lennox LB tablet with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Convenient small package to eliminate weighing
  • Fast preparation – no stirring required

Preparation Note

Add each 1.68 g tablet to 48.3 mL water (no stirring required). Autoclave for 20 minutes at 121 °C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Other Notes

13.72g/L Agar
9.14g/L Tryptone
4.57 g/L Yeast Extract
4.57 g/L NaCl
1.6g/L inert binding agents

Legal Information

Zeocin is a registered trademark of Cayla Sarl

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

L7025-100TAB: + L7025-BULK: + L7025-VAR: + L7025-500TAB:

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Certificates of Analysis (COA)

Lot/Batch Number
0000531907
0000531905
0000531905
0000531907
0000453740

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Shun Gen Huang et al.
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Surgical intervention-related trauma contributes largely to the development of postoperative immunosuppression, with reduced resistance to secondary bacterial infection. This study compared the impact of laparotomy versus laparoscopy on macrophage-associated bactericidal ability and examined whether laparotomy renders the host more susceptible
Atlas, R. M., L.C. Parks
Handbook of Microbiological Media (1993)
Wenjun Kong et al.
Nature protocols, 15(3), 750-772 (2020-02-14)
Single-cell technologies are offering unparalleled insight into complex biology, revealing the behavior of rare cell populations that are masked in bulk population analyses. One current limitation of single-cell approaches is that lineage relationships are typically lost as a result of

Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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