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Merck

M2003

MTT Formazan

powder

Synonym(s):

1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan

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About This Item

Empirical Formula (Hill Notation):
C18H17N5S
CAS Number:
57360-69-7
Molecular Weight:
335.43
NACRES:
NA.47
PubChem Substance ID:
24896591
UNSPSC Code:
12171500
MDL number:
MFCD00080731

Key Documents

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InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+

SMILES string

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI key

GIFKVQOPAJDJLO-SZVLODPRSA-N

assay

≥97% (TLC)

form

powder

color

purple

solubility

acetone: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

MTT Formazan has been used to measure cell viability.

Biochem/physiol Actions

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

M2003-500MG: + M2003-VAR: + M2003-1G: + M2003-250MG: + M2003-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
0000325975
0000325975
0000210043
0000210043
SHBQ7917

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