MRCKα Protein, active, 250 µg

55.3kDa

FUNCTION: SwissProt: Q5VT25 # May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
COFACTOR: Magnesium.
SIZE: 1732 amino acids; 197307 Da
SUBUNIT: Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Note=Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity).
TISSUE SPECIFICITY: Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
SIMILARITY: SwissProt: Q5VT25 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 CNH domain. & Contains 1 CRIB domain. & Contains 1 PH domain. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain.

Also available in 10 μg size (cat#14-691) and in bulk (cat# 14-691-K).

6 months at -70°C from date of shipment

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

routinely evaluated by phosphorylation of S6 Kinase/Rsk2 Substrate Peptide 2 (KKRNRTLTV)

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