G7663
ゼラチンブロッキングバッファー
for Western blotting, powder blend
別名:
blocking buffer
製品名
ゼラチンブロッキングバッファー, for Western blotting, powder blend
grade
Molecular Biology
form
powder blend
storage temp.
room temp
Quality Level
Application
ナイロン・ニトロセルロ-スを使用するサザンブロット法やウェスタンブロット法に適しています。
Preparation Note
5~-10分で溶解します。ろ過や加熱を行うことなく、淡黄色の溶液となります。
General description
Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).
保管分類
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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プロトコル
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.
関連コンテンツ
Instructions
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