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Merck

L6669

LuminoCt® qPCR ReadyMix™

For fast probe-based quantitative PCR

別名:

LuminoCt®

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この商品について

NACRES:
NA.55
UNSPSC Code:
41106300

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製品名

LuminoCt® qPCR ReadyMix™, For fast probe-based quantitative PCR

form

liquid

usage

sufficient for 100 reactions
 sufficient for 2000 reactions
 sufficient for 500 reactions

feature

dNTPs included
hotstart

technique(s)

qPCR: suitable

color

colorless

input

purified DNA

suitability

suitable for (quantitative PCR)

application(s)

agriculture

compatibility

Bio-Rad MyiQ ( )
 Bio-Rad iCycler iQ
 Bio-Rad iQ 5
for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

detection method

probe-based

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

関連するカテゴリー

Application

LuminoCt® qPCR ReadyMix™ has been used in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay to quantify the reverse transcription product or cDNA for gene expression analysis. It has also been used in qPCR for quantifying the RNA extracted from rhinoviruses.

Features and Benefits

  • Assay results in as little as 25 minutes
  • Designed to deliver unsurpassed accuracy, precision, and sensitivity
  • Extensive design and validation of ReadyMix chemistry virtually eliminates the need to optimize assay parameters
  • Ideal for rapid, high-throughput 2-step qPCR protocols
  • The master mix allows consistency and reproducibility from one reaction to the next
  • Reduced preparation time and the risk of contamination from multiple pipetting steps
  • Compatible with commonly available real-time instrument platforms for probe-based qPCR assays
  • Require small amplicons (<200 bp) for optimal results
  • Compatible with commercial primer sets, including TaqMan® assays
  • JumpStart™ Taq DNA polymerase allows room temperature set-up and the hot-start mechanism prevents primer-dimer and non-specific product formation

General description

Inclusion of JumpStart™ Taq antibody in the ReadyMix™ eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.

  • Kit is designed to perform probe-based qPCR assays on commonly available real-time instrument platforms.
  • ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays.
  • Kit is not compatible with qPCR instruments that utilize glass capillary tubes.
LuminoCt® qPCR ReadyMix combines the performance enhancements of our JumpStart™ Taq antibody for hot start PCR with the convenience of an easy-to-use reaction mixture.

Other Notes

  • Commercially available 5′ nuclease assays (e.g., TaqMan® Assays) are compatible with LuminoCt ReadyMix.
  • At a minimum, use software to design primers and probes for assays and ensure that qPCR amplicons are <200 bp.
  • Sigma recommends designing primers and probes with Beacon Designer software

To order primers and probes from Sigma, visit www.sigma.com/oligos

Packaging

LuminoCt qPCR ReadyMix, Catalog Number L5794, contains optimized concentrations of Tris-HCl, pH 8.3, KCl, dNTPs (dATP, dCTP, dGTP, TTP), stabilizers, MgCl2 and JumpStart Taq DNA Polymerase. Provided as 100, 500 and 2000 reactions (25 μL mix in a 50 μL reaction volume).

100x ROX internal reference dye, Catalog Number R4526. Optional, for use with machines compatible with an internal reference dye, e.g., ABI and Stratagene.

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL

Legal Information

Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
LuminoCt is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

キットの構成要素は別途購入可能です。

製品番号
詳細
SDS
  • R4526100X ROX internal reference dyeSDS

保管分類

10 - Combustible liquids

wgk

WGK 1

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試験成績書（COA）

Lot/Batch Number
0000453142
0000453142
0000444133
0000444015
0000444017

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Samer M Hussein et al.
Nature, 471(7336), 58-62 (2011-03-04)
The mechanisms underlying the low efficiency of reprogramming somatic cells into induced pluripotent stem (iPS) cells are poorly understood. There is a clear need to study whether the reprogramming process itself compromises genomic integrity and, through this, the efficiency of
Laura de Diego-García et al.
Biochimica et biophysica acta. Molecular basis of disease, 1863(1), 43-51 (2016-10-22)
The Ubiquitin-Proteasome System (UPS) is essential for the regulation of the cellular proteostasis. Indeed, it has been postulated that an UPS dysregulation is the common mechanism that underlies several neurological disorders. Considering that extracellular nucleotides, through their selective P2Y2 receptor
Philip Owens et al.
PloS one, 8(6), e67533-e67533 (2013-07-11)
Bone Morphogenetic Proteins (BMPs) are secreted cytokines that are part of the Transforming Growth Factor β (TGFβ) superfamily. BMPs have been shown to be highly expressed in human breast cancers, and loss of BMP signaling in mammary carcinomas has been
J Martínez et al.
Letters in applied microbiology, 50(6), 603-610 (2010-04-22)
To develop a real-time PCR-based strategy for the detection of Paenibacillus larvae vegetative cells and spores to improve the diagnosis and the screening of American foulbrood (AFB), the most harmful pathology of honeybee brood. A real-time PCR that allowed selective
Judit Abdai et al.
Frontiers in psychology, 7, 1772-1772 (2016-11-30)
Social evaluation is a mental process that leverages the preference toward prosocial partners (positivity bias) against the avoidance of antisocial individuals (negativity bias) in a cooperative context. The phenomenon is well-known in humans, and recently comparative investigations looked at the
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資料

PCR Master Mix

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

Probe-based qPCR

Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.

PCRマスターミックス

PCRマスターミックスは、DNAポリメラーゼ、dNTP、MgCl2、バッファーのような成分によりPCR/RT-PCRの反応を簡略化するもので、市販のものも自身で作製するものもあります。

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プロトコル

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

関連コンテンツ

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

RT-qPCR – 定量的逆転写PCR

RT-qPCRは、遺伝子発現や病原体検出における用途で、特定のターゲットを検出します。

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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