06-942-AF555
Anti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor™ 555 Conjugate
from rabbit, ALEXA FLUOR™ 555
別名:
H3K9ac, Histone H3, Lys9-acetylated, H3 histone family, member T, Lys9-acetylated, Lys9-acetylated histone 3, histone cluster 3, Lys9-acetylated, Lys9-acetylated H3
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 555
Clone:
polyclonal
Application:
FACS, ICC
Citations:
-
biological source
rabbit
Quality Segment
conjugate
ALEXA FLUOR™ 555
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse, human
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
acetylation (Lys9)
Gene Information
human ... HIST1H3F(8968)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
~17 kDa observed.
Immunogen
KLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of histone H3 with acetylated Lys9.
Application
Anti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor™ 555 Conjugate, Cat. No. 06-942-AF555, is a highly specific rabbit polyclonal antibody that targets histone H3 Lys9 acetylation (H3K9ac) and has been tested in Flow Cytometry and Immunocytochemistry.
Flow Cytometry Analysis: 2 µL from a representative lot detected cell population with histone H3 Lys9 acetylation (H3K9ac) among one million HEK293 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected nuclear histone H3 Lys9 acetylation (H3K9ac) in A431, HeLa, and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 06-492) is shown to be suitable also for Western blotting, dot blot, flow cytometry, chromatin immunoprecipitation (ChIP) and ChIP-seq applications.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected nuclear histone H3 Lys9 acetylation (H3K9ac) in A431, HeLa, and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 06-492) is shown to be suitable also for Western blotting, dot blot, flow cytometry, chromatin immunoprecipitation (ChIP) and ChIP-seq applications.
Physical form
Purified rabbit polyclonal antibody in PBS with 1.5% BSA with 0.05% sodium azide.
Analysis Note
Evaluated by Immunocytochemistry in HUVECs.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclear histone H3 Lys9 acetylation (H3K9ac) in HUVECs.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclear histone H3 Lys9 acetylation (H3K9ac) in HUVECs.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
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12 - Non Combustible Liquids
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