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Merck

07-819

Anti-phospho-EGFR (Tyr1148) Antibody

Upstate®, from rabbit

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human
Application:
WB
Citations:
9
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biological source

rabbit

Quality Segment

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pTyr1148)

Gene Information

human ... EGFR(1956)

General description

185 kDa

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-EGFR (Tyr1148)

Application

Immunoblot Analysis: A 1:1000 dilution of this lot detected phospho-EGFR (Tyr1148) in RIPA lysates from EGF stimulated A431 cells.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
This Anti-phospho-EGFR (Tyr1148) Antibody is validated for use in WB for the detection of phospho-EGFR (Tyr1148).

Biochem/physiol Actions

Predicted to cross-react with mouse and rat based on sequence homology (100%), but have not been tested.
Recognizes phospho-EGFR (Tyr1148)

Physical form

100 μl of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
ImmunoAffinity Purified

Preparation Note

Stable for 2 years at -20°C from date of shipment.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Analysis Note

Routinely evaluated by immunoblot.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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保管分類

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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