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Merck

07-819

Anti-phospho-EGFR (Tyr1148) Antibody

Upstate®, from rabbit

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human
Application:
WB
Citations:
9
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biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pTyr1148)

Quality Level

Gene Information

human ... EGFR(1956)

General description

185 kDa

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-EGFR (Tyr1148)

Application

Immunoblot Analysis: A 1:1000 dilution of this lot detected phospho-EGFR (Tyr1148) in RIPA lysates from EGF stimulated A431 cells.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
This Anti-phospho-EGFR (Tyr1148) Antibody is validated for use in WB for the detection of phospho-EGFR (Tyr1148).

Biochem/physiol Actions

Predicted to cross-react with mouse and rat based on sequence homology (100%), but have not been tested.
Recognizes phospho-EGFR (Tyr1148)

Physical form

100 μl of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
ImmunoAffinity Purified

Preparation Note

Stable for 2 years at -20°C from date of shipment.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Analysis Note

Routinely evaluated by immunoblot.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

07-819:

jan


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Polymorphonuclear leukocytes-mediated lysis of A431 cells induced by IgG1 mouse anti-epidermal growth factor receptor monoclonal antibodies.
Kawamoto, T, et al.
In Vitro Cellular & Developmental Biology, 28A, 782-786 (1992)
Changes in retinal cell fate induced by overexpression of EGF receptor
Lillien, L.
Nature, 377, 158-162 (1995)
Wojciech Stec et al.
Oncotarget, 9(9), 8560-8572 (2018-03-02)
Despite intensive research no therapies targeted against the oncogenic EGFRvIII are present in the clinic. One of the reasons is the elusive nature of the molecular structure and activity of the truncated receptor. The recent publications indicate the EGF-bound wild-type
G R Johnson et al.
The Journal of biological chemistry, 268(4), 2924-2931 (1993-02-05)
The COOH-terminal half of the amphiregulin (AR) molecule has sequence homology to epidermal growth factor (EGF). The ability of AR to elicit in vivo phosphorylation of the EGF receptor (EGFR) and p185erbB2 was studied in four human epithelial cell lines
Cytotoxic properties of DAB486EGF and DAB389EGF, epidermal growth factor (EGF) receptor-targeted fusion toxins.
Shaw, J P, et al.
The Journal of Biological Chemistry, 266, 21118-21124 (1991)

グローバルトレードアイテム番号

カタログ番号GTIN
07-81904053252268533

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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