07-1507
Anti-phospho-Mypt1 (Ser507) Antibody
from rabbit, purified by affinity chromatography
別名:
Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
species reactivity (predicted by homology)
human (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer507)
Gene Information
human ... PPP1R12A(4659)
General description
Myosin phosphatase targeting subunit 1 (MYPT1) is a regulatory targeting subunit that is thought to be responsible for targeting the holoenzyme to the proper positions in muscle and moving PP1 to myosin. MYPT1 is the major subunit involved in myosin light chain phosphatase (MLCP). It is also essential for vasodilation caused by nitric oxide (NO). A decrease in MYPT1 has been shown to play a role in increased tolerance of NO in pulmonary arteries.
~120 kDa observed.
Uniprot gives a calculated molecular weight of 115 kDa, which can be observed at higher molecular weights due to hyperphosphorylation. Uncharacterized bands appear at ~30 kDa and ~130 kDa in some lysates.
Uniprot gives a calculated molecular weight of 115 kDa, which can be observed at higher molecular weights due to hyperphosphorylation. Uncharacterized bands appear at ~30 kDa and ~130 kDa in some lysates.
Immunogen
Epitope: Phosphorylated (Ser507)
KLH-conjugated linear peptide corresponding to human Mypt1 phosphorylated at (Ser507).
Application
Research Category
Signaling
Cell Structure
Signaling
Cell Structure
Research Sub Category
Developmental Signaling
Muscle Physiology
Developmental Signaling
Muscle Physiology
This Anti-phospho-Mypt1 (Ser507) Antibody is validated for use in WB for the detection of phospho-Mypt1 (Ser507).
Biochem/physiol Actions
Other homologies: Rat (89% sequence homology).
This antibody recognizes Mypt1 phosphorylated at (Ser507).
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Untreated and PMA-treated NIH/3T3 cell lysates
Untreated and PMA-treated NIH/3T3 cell lysates
Evaluated by Western Blot in untreated and PMA-treated NIH/3T3 cell lysates.
Western Blot Analysis: 2 µg/mL of this antibody detected Mypt1 in 10 µg of untreated and PMA-treated NIH/3T3 cell lysates.
Western Blot Analysis: 2 µg/mL of this antibody detected Mypt1 in 10 µg of untreated and PMA-treated NIH/3T3 cell lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
12 - Non Combustible Liquids
wgk
WGK 1