Anti-NCoA-2 Antibody

NCoA2 (nuclear receptor coactivator 2) is a transcriptional coactivator for steroid receptors and nuclear receptors. Also known as glucocorticoid receptor interacting protein 1 (GRIP1), steroid receptor coactivator-2 (SRC-2), or transcriptional mediators/intermediary factor 2 (TIF2), this coregulatory protein contains several nuclear receptor interacting domains and an intrinsic histone acetyltransferase activity. NCoA-2 assists nuclear receptors in the upregulation of DNA expression. In cells ligand activated nuclear receptors recruit NCoA-2 to DNA where it acylates histones to make targeted regions accessible to transcriptional machinery.

~ 160 kDa

Epitope: Unknown

Linear peptide corresponding to human NCoA-2

Chromatin Immunoprecipitation:
A representative lot of this antibody was used for chIP. See Monroe, D.G., et al. (2006) J. Biol. Chem. 281(39):28596-28604.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-NCoA-2 Antibody (rabbit polyclonal antibody) validated in WB to detect NCoA-2 also known as Transcriptional intermediary factor 2, glucocorticoid receptor-interacting protein-1, nuclear receptor coactivator 2.

Homology to mouse and rat is 95%.

This antibody recognizes NCoA-2.

Affinity purified

Format: Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
U2 OS cell lysate

Evaluated by Western Blot in U2 OS cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected SRC2 on 10 µg of U2 OS cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.