09-272
Anti-phospho-p38α (Thr180/Tyr182) Antibody
Upstate®, from rabbit
別名:
SAPK2a, MAPK14, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, p38ALPHA, mitogen-activated protein kinase 14
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
mouse, rat, human
Application:
immunoprecipitation (IP)
western blot
western blot
Technique(s):
immunoprecipitation (IP): suitable
western blot: suitable
western blot: suitable
Citations:
28
Uniprot accession no.:
製品名
Anti-phospho-p38α (Thr180/Tyr182) Antibody, Upstate®, from rabbit
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
packaging
antibody small pack of 25 μL
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pThr180/pTyr182)
Quality Level
Gene Information
human ... MAPK14(1432)
関連するカテゴリー
Analysis Note
Control
Anisomycin treated HeLa cells
Anisomycin treated HeLa cells
Routinely evaluated by immunoblot.
Application
Immunoblot Analysis: A 1:1,000 dilution of the lot detected p38α dually phosphorylated on residues Thr180 and Tyr182 in lysate from HeLa cells stimulated with anisomycin.
Immunfluorescence: A 1:100 dilution of this antibody detected p38α phosphorylated on both residues, Thr180 and Tyr182, in fixed HeLa cells stimulated with anisomycin.
Immunoprecipitation (IP): 2-5 μL (5-10 μL/mL) of this antibody immunoprecipitated both active p38α from anisomycin treated HeLa whole cell extract and human recombinant GST-p38α, separately, in RIPA buffer.
Immunoprecipitation Kinase Reaction (IPK): 2-5 μL of this lot immunoprecipitated phosphorylated p38α from both anisomycin treated HeLa cells as well as recombinant p38α, separately, and then placed into an in vitro Kinase reaction with recombinant GST-ATF-2 where it phosphorylated ATF-2 on Thr69/71.
Immunfluorescence: A 1:100 dilution of this antibody detected p38α phosphorylated on both residues, Thr180 and Tyr182, in fixed HeLa cells stimulated with anisomycin.
Immunoprecipitation (IP): 2-5 μL (5-10 μL/mL) of this antibody immunoprecipitated both active p38α from anisomycin treated HeLa whole cell extract and human recombinant GST-p38α, separately, in RIPA buffer.
Immunoprecipitation Kinase Reaction (IPK): 2-5 μL of this lot immunoprecipitated phosphorylated p38α from both anisomycin treated HeLa cells as well as recombinant p38α, separately, and then placed into an in vitro Kinase reaction with recombinant GST-ATF-2 where it phosphorylated ATF-2 on Thr69/71.
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
This Anti-phospho-p38α (Thr180/Tyr182) Antibody is validated for use in IP, WB for the detection of phospho-p38α (Thr180/Tyr182).
Biochem/physiol Actions
Recognizes p38α dual phosphorylation of Thr180 and Tyr182.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
38 kDa
Immunogen
Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-p38α (Thr180/Tyr182).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB3828
Physical form
ImmunoAffinity Purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Anil K Kamaraju et al.
The Journal of biological chemistry, 280(2), 1024-1036 (2004-11-03)
TGF-beta is a multifunctional cytokine known to exert its biological effects through a variety of signaling pathways of which Smad signaling is considered to be the main mediator. At present, the Smad-independent pathways, their interactions with each other, and their
Zhi-Ling Shan et al.
Molecular medicine reports, 16(3), 3055-3060 (2017-07-18)
Acute promyelocytic leukemia (APL) is a special subtype of acute myeloid leukemia that responds to treatment with all‑trans retinoic acid and arsenic trioxide. However, severe side effects and drug resistance limit the effectiveness of these treatments. Hence, new drugs for
A high-quality secretome of A549 cells aided the discovery of C4b-binding protein as a novel serum biomarker for non-small cell lung cancer.
Xiaoyang Luo,Yansheng Liu,Rui Wang,Haichuan Hu,Rong Zeng,Haiquan Chen
Journal of proteomics null
Manon Ruffin et al.
American journal of physiology. Lung cellular and molecular physiology, 314(4), L635-L641 (2018-01-20)
Cystic fibrosis patients exhibit chronic Pseudomonas aeruginosa respiratory infections and sustained proinflammatory state favoring lung tissue damage and remodeling, ultimately leading to respiratory failure. Loss of cystic fibrosis transmembrane conductance regulator (CFTR) function is associated with MAPK hyperactivation and increased
Simon C Chow et al.
International journal of cancer, 136(6), 1341-1350 (2014-08-02)
Surgery is required for the curative treatment of lung cancer but is associated with high rates of postoperative pneumonias predominantly caused by gram negative bacteria. Recent evidence suggests that these severe infectious complications may decrease long term survival after hospital
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 09-272 | 04053252736711 |
| 09-272-25UL | 04054839342547 |
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