Anti-TKS5 (SH3 #1) Antibody

130-150 kDa Uncharacterized bands(s) may be observed in some cell lysates.

Tks5 (previously known as Fish), is a large scaffolding protein with one amino-terminal PX domain and five SH3 domains. It is cytoplasmic in normal fibroblasts. In Src-transformed cells, Tks5 localizes to podosomes. The coexpression of Tks5 and constitutively active Src in epithelial cancer cells resulted in the appearance of podosomes/invadopodia. Cells with reduced Tks5 expression are poorly invasive through matrigel. Tks5 is expressed and localized to podosomes/invadopodia in some invasive human cancer cell lines and tumor tissue, particularly breast cancers and melanomas. Thus, Tks5 appears to be required for podosome/invadopodia formation and for invasion of some cancer cells.

Epitope: 166-225 aa

Recombinant Protein, aa: 166-225. Homology: Human, Dog, Horse, Rat, Cow (100%, 60/60); Chicken (93%, 56/60)

Anti-TKS5 (SH3 #1) Antibody detects level of TKS5 (SH3 #1) & has been published & validated for use in WB, IC & IP.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Western Blot Analysis: Antibody detected TKS5 in 20 μg of NIH-3T3 (100% confluent) lysate.

Other homologies: Human, Canine, Equine, Rat, and Bovine (100% sequence homology), Chicken (93% sequence homology)

Format: Purified

Protein A purified

Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Control
Western Blot::
NIH-3T3 (100% confluent) lysate
Immunocytochemistry:
Mouse 3T3-Src(Y527F) cells

Routinely evaluated by Western Blot on NIH-3T3 (100% confluent) lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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