Anti-PI3 Kinase Antibody, p110α

110 kDa

Phosphoinositol-3-kinases (PI3 Kinase, PI3K) are a family of lipid kinases that mediate many intracellular signaling responses in both physiological and pathophysiological states. PI3 Kinase is a heteroduplex with three classes (I, II, & III) of catalytic domain. The class I PI3 Kinases are made up of a p85 regulatory protein and a p110 catalytic domain. There are 4 isoforms of the class I p110 catalytic domains. Class I(A) include α, β, γ and class I(B). They are activated downstream via receptor tyrosine kinases or G-protein coupled receptors. Once activated, PI3K generates phosphatidylinositol 3,4,5-trisphosphate (PIP3) from PIP2. It was later found that PTEN, a lipid phosphatase, acts opposite to PI3K by dephosphorylating PIP3, converting it to PIP2, thus de-activating the effects of PI3K. Increased levels of PIP3 in the cell leads to the activation of many key signaling regulators including Akt and PDK as well as calcium release. One of the primary roles of PI3K is its regulation of cell growth and cell metabolism. Both of these play a critical role in cancer as some believe that a tumor’s shift to glycolysis is due to the activation of PI3K. PI3 kinases have been linked with numerous disease states, including cancer, diabetes, allergic response, hypertension, cardiac contractility, atherosclerosis, sepsis and autoimmune/ inflammatory disorders.

Epitope: Internal

KLH-conjugated synthetic linear peptide corresponding to an internal region of class I catalytic domain of PI3 Kinase that is specific to the p110 alpha isoform.

Detect PI3 Kinase using this Anti-PI3 Kinase Antibody, p110α validated for use in WB, IP, IH, ELISA.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Recognizes PI3 Kinase p110α.

Immunogen Affinity Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Stable for 1 year at 2-8ºC from date of receipt.

Evaluated by western blot on Jurkat cell extract.

Western Blotting Analysis: 0.5 µg/mL (1:2,000) dilution of this antibody detected PI3K, p110α in Jurkat cell extract.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-399

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