form
medium granules
Quality Segment
manufacturer/tradename
GranuCult®
technique(s)
microbiological culture: suitable
pH
7.5-7.9 (25 °C, 75 g/L in H2O)
solubility
75 g/L
application(s)
clinical
food and beverages
microbiology
pathogen testing
storage temp.
20-25°C
suitability
Salmonella spp. (spp.)
General description
for the detection and isolation of pathogenic intestinal bacteria
Analysis Note
Appearance (clearness):clear
Appearance (colour):blue-green
pH-value (25 °C):7.5 - 7.9
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)):+
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)):+
Growth (Shigella flexneri ATCC 12022 (WDCM 00126)):+
Growth (Shigella sonnei ATCC 11060):+
Growth (Proteus mirabilis ATCC 14273):+
Growth (Escherichia coli ATCC 25922 (WDCM 00013)):+
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)):-
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)):inhibited
Colony colour (Salmonella typhimurium ATCC 14028 (WDCM 00031)):blue-green
Colony colour (Salmonella enteritidis ATCC 13076 (WDCM 00030)):blue-green
Colony colour (Shigella flexneri ATCC 12022 (WDCM 00126)):green to blue-green
Colony colour (Shigella sonnei ATCC 11060):green to blue-green
Colony colour (Proteus mirabilis ATCC 14273):green to blue-green
Colony colour (Escherichia coli ATCC 25922 (WDCM 00013)):orange-red
Colony colour (Enterococcus faecalis ATCC 29212 (WDCM 00087)):orange
Black centre (Salmonella typhimurium ATCC 14028 (WDCM 00031)):+
Black centre (Salmonella enteritidis ATCC 13076 (WDCM 00030)):+
Black centre (Shigella flexneri ATCC 12022 (WDCM 00126)):-
Black centre (Shigella sonnei ATCC 11060):-
Black centre (Proteus mirabilis ATCC 14273):+/-
Black centre (Escherichia coli ATCC 25922 (WDCM 00013)):-
Precipitate (Salmonella typhimurium ATCC 14028 (WDCM 00031)):-
Precipitate (Salmonella enteritidis ATCC 13076 (WDCM 00030)):-
Precipitate (Shigella flexneri ATCC 12022 (WDCM 00126)):-
Precipitate (Shigella sonnei ATCC 11060):-
Precipitate (Proteus mirabilis ATCC 14273):-
Precipitate (Escherichia coli ATCC 25922 (WDCM 00013)):+
Incubation: 18-24 hours at 35-37°C, aerobic
The requirement growth „inhibited" for strain Enterococcus feacalis ATCC 19433 can entail a complete inhibition of growth. In this case the assessment of the colony color is not possible and it is reported as divergent.
Appearance (colour):blue-green
pH-value (25 °C):7.5 - 7.9
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)):+
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)):+
Growth (Shigella flexneri ATCC 12022 (WDCM 00126)):+
Growth (Shigella sonnei ATCC 11060):+
Growth (Proteus mirabilis ATCC 14273):+
Growth (Escherichia coli ATCC 25922 (WDCM 00013)):+
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)):-
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)):inhibited
Colony colour (Salmonella typhimurium ATCC 14028 (WDCM 00031)):blue-green
Colony colour (Salmonella enteritidis ATCC 13076 (WDCM 00030)):blue-green
Colony colour (Shigella flexneri ATCC 12022 (WDCM 00126)):green to blue-green
Colony colour (Shigella sonnei ATCC 11060):green to blue-green
Colony colour (Proteus mirabilis ATCC 14273):green to blue-green
Colony colour (Escherichia coli ATCC 25922 (WDCM 00013)):orange-red
Colony colour (Enterococcus faecalis ATCC 29212 (WDCM 00087)):orange
Black centre (Salmonella typhimurium ATCC 14028 (WDCM 00031)):+
Black centre (Salmonella enteritidis ATCC 13076 (WDCM 00030)):+
Black centre (Shigella flexneri ATCC 12022 (WDCM 00126)):-
Black centre (Shigella sonnei ATCC 11060):-
Black centre (Proteus mirabilis ATCC 14273):+/-
Black centre (Escherichia coli ATCC 25922 (WDCM 00013)):-
Precipitate (Salmonella typhimurium ATCC 14028 (WDCM 00031)):-
Precipitate (Salmonella enteritidis ATCC 13076 (WDCM 00030)):-
Precipitate (Shigella flexneri ATCC 12022 (WDCM 00126)):-
Precipitate (Shigella sonnei ATCC 11060):-
Precipitate (Proteus mirabilis ATCC 14273):-
Precipitate (Escherichia coli ATCC 25922 (WDCM 00013)):+
Incubation: 18-24 hours at 35-37°C, aerobic
The requirement growth „inhibited" for strain Enterococcus feacalis ATCC 19433 can entail a complete inhibition of growth. In this case the assessment of the colony color is not possible and it is reported as divergent.
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
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