1.12018
Albumin fraction V
(from bovine serum) for biochemistry
別名:
Albumin fraction V, Bovine serum albumin, Serum albumin
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この商品について
Biological source:
bovine
Form:
solid
Technique(s):
immunoassay: suitable
western blot: suitable
western blot: suitable
Concentration:
≥97% protein (spectralphotometric; calc. on anhydrous substance)
≥98% (Assay of Albumin (Agarose gel electrophoresis))
≥98% (Assay of Albumin (Agarose gel electrophoresis))
製品名
Albumin fraction V, (from bovine serum) for biochemistry
biological source
bovine
form
solid
concentration
≥97% protein (spectralphotometric; calc. on anhydrous substance)
≥98% (Assay of Albumin (Agarose gel electrophoresis))
technique(s)
immunoassay: suitable
western blot: suitable
pH
6.8-7.2 (20 °C, 1 g/L in H2O)
solubility
1 g/L
bulk density
150 kg/m3
storage temp.
2-8°C
Analysis Note
Appearance (colour): slightly yellow
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable
Application
Albumin fraction V has been used:
- as a component of perfusion buffer for perfusion protocol for rodent organ perfusion
- as a blocking agent in western blot analysis
- as a nitrated protein standard to study the effects of antibodies against 3-nitrotyrosine and to characterize the binding and detection of nitrated proteins in one-sided and sandwich immunoassays
Biochem/physiol Actions
Bovine Serum Albumin (BSA) is the chief component of cell culture media and also works as a blocking agent in enzyme-linked immunosorbent assay (ELISA). It is beneficial for embryonic stem cells (hESC) differentiation and assists the transport of drugs, hormones, and fatty acids.
General description
Bovine serum albumin (BSA) belongs to the serum albumin family with three domains and two sub-domains each. This globular, α-helical, non-glycosylated protein has 17-disulfide bonds.
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
J L Lanciego et al.
Brain research. Brain research protocols, 2(4), 323-332 (1998-06-19)
The present report deals with a multiple tract-tracing procedure in non-human primates enabling the simultaneous visualization of retrogradely transported Fluoro-Gold (FG) and cholera toxin B subunit (CTB) in combination with anterogradely transported biotinylated dextran amine (BDA). Two issues have played
Holger Jungbluth et al.
Clinical oral investigations, 18(9), 2077-2086 (2014-02-08)
The aim of this series of studies was the development and validation of a new model for evaluation of dentinal hypersensitivity (DH) therapies. Roots from extracted human teeth were sealed with a flowable composite. In the cervical area, a 3-mm-wide
Sebastian Ivens et al.
Brain : a journal of neurology, 130(Pt 2), 535-547 (2006-11-24)
It has long been recognized that insults to the cerebral cortex, such as trauma, ischaemia or infections, may result in the development of epilepsy, one of the most common neurological disorders. Human and animal studies have suggested that perturbations in
Thomas Franze et al.
The Analyst, 129(7), 589-596 (2004-06-24)
Three monoclonal antibodies (mAb) and three polyclonal antibodies (pAb) have been characterized and compared with respect to their cross-reactivities and affinities for 3-nitrotyrosine, eight aromatic compounds with similar chemical structures, a peptide containing a single nitrotyrosine residue, and fourteen nitrated
Calcitriol Inhibits Proliferation and Potentially Induces Apoptosis in B16-F10 Cells.
Sutedja, et al.
Medical Science Monitor Basic Research, 28, e935139-e935139 (2022)
資料
BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 1.12018.0100 | 04022536138367 |
| 1.12018.0025 | 04022536138350 |
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