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1.50252

Purospher® STAR RP-18 endcapped (5 µm) HPLC Columns

L × I.D. 250 mm x 4 mm LiChroCART™ HPLC cartridge, manuCART™ holder required for use

別名:

Purospher® STAR RP-18 endcapped (5 µm) LiChroCART™ 250-4

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この商品について

UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
5 μm
Matrix active group:
C18 (RP18), C18 bonding phase
Pore size:
120 Å pore size
Matrix:
fully porous particle
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製品名

Purospher® STAR RP-18 endcapped (5 µm), L × I.D. 250 mm x 4 mm LiChroCART™ HPLC cartridge, manuCART™ holder required for use

material

stainless steel column

Quality Segment

agency

suitable for USP L1

product line

Purospher®

feature

endcapped

analyte functional class(es)

antibiotics, explosives, fat soluble vitamins, lipids, nutraceuticals and antioxidants, oils, pigments, sweeteners, water soluble vitamins

analyte chemical class(es)

PAHs, PCBs (aroclor identification), PFAS, aflatoxins, alcohols / fermentation, alcohols and aldehyde, aliphatic amines, alkaloids, amino acids, aromatic amines, cannabinoids as tms derivatives, carotenoids, catecholamines, coloring compounds, essential oils, esters, fatty acids, flavonoids, glycans, ketones, metabolized steroids, nitrosamines, nucleic acids, nucleosides, oligonucleotides, organic phosphates, peptides, pesticides, phenols, phospholipids, phthalate esters, preservatives, proteins, steroids (cholesteryl esters), sulfonamides

packaging

1 ea of

extent of labeling

17% Carbon loading

parameter

250 bar pressure, 65 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

column L × I.D.

25 cm × 4 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

C18 (RP18), C18 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase

storage temp.

no temp limit

General description

Purospher® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher® STAR RP-18 endcapped, 5 µm HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.Purospher® STAR RP-18 endcapped, 5 µm LiChroCART™ 250-4 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001. The manuCART™ can be used to hold one cartridge column with or without direct integration of a 4-4 mm guard column. The recommended guard column is Purospher® STAR RP-18 endcapped (5 µm) LiChroCART™ 4-4 part number 1.50250.0001. LiChroCART™ cartridge columns mounted with a manuCART™ cartridge column holder are hand-tight only and have therefore a maximum pressure stability of 250 bar. For higher pressure stability requirements Purospher® STAR RP-18 endcapped, 5 µm Hibar® RT 250-4 HPLC columns are available part number 1.150037.0001.The column dimension of 250 mm length x 4 mm I.D. is the optimal choice for high resolution HPLC separation of complex samples.Item 1.50252.1003 is a Validation kit containing 3 Purospher® STAR RP-18 endcapped, 5 µm LiChroCART™ 250-4 cartridge columns from 3 different base material lots.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended for the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat-soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water-soluble vitamins.

Features and Benefits

  • Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 130 bar
Theoretical Plates (N/m) (Toluene): ≥ 80000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): ≥ 1.5

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

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