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Merck

1.50432

LiChrosorb® RP-8 (5 µm) HPLC Columns

L x I.D. 125 mm x 4 mm, Hibar® RT HPLC Column

別名:

TM=["LiChrosorb"] C8 HPLC column

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この商品について

UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
5 μm
Matrix active group:
C8 (RP8), C8 bonding phase
Pore size:
100 Å pore size
Matrix:
fully porous particle
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製品名

LiChrosorb® RP-8 (5 µm) Hibar® RT 125-4, suitable for HPLC

material

stainless steel column

Quality Segment

agency

suitable for USP L7

product line

LiChrosorb®

feature

endcapped: no

analyte functional class(es)

antibiotics, fat soluble vitamins, lipids, nutraceuticals and antioxidants, oils, pigments

analyte chemical class(es)

PCBs (aroclor identification), PFAS, aflatoxins, alcohols and aldehyde, alkaloids, amino acids, cannabinoids as tms derivatives, carotenoids, coloring compounds, essential oils, esters, fatty acids, flavonoids, ketones, metabolized steroids, nitrosamines, nucleosides, oligonucleotides, peptides, pesticides, phenols, phospholipids, phthalate esters, preservatives, proteins, steroids (cholesteryl esters), sulfonamides

parameter

400 bar pressure, 60 °C max. temp.

technique(s)

HPLC: suitable, mass spectrometry (MS): suitable (Low)

column L × I.D.

12.5 cm × 4 mm

matrix

fully porous particle

matrix active group

C8 (RP8), C8 bonding phase

particle size

5 μm

pore size

100 Å pore size

operating pH range

2-7.5

separation technique

reversed phase, supercritical fluid (SFC)

storage temp.

no temp limit

General description

HPLC column
LiChrosorb® RP-8 is a reliable irregular shaped silica gel carriers with reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds.This sorbent is packed into a Hibar 125-4 HPLC column. For mounting of a LiChroCART 4-4 guard column, the guard column holder 1.51487 has to be used.

Application


  • Determination of amphotericin B, liposomal amphotericin B, and amphotericin B colloidal dispersion in plasma by high-performance liquid chromatography.: This study by Egger et al. focuses on the HPLC determination of various forms of amphotericin B in plasma, utilizing LiChrosorb® RP-8 as the stationary phase. This method ensures accurate monitoring of these antifungal agents in clinical settings (Egger et al., 2001).

  • Determination of peptide hydrophobicity parameters by reversed-phase high-performance liquid chromatography.: This research by Rothemund et al. used LiChrosorb® RP-8 to determine the hydrophobicity of various peptides, providing valuable insights for peptide characterization in biochemical research (Rothemund et al., 1994).

  • HPLC determination of doxorubicin, doxorubicinol and four aglycone metabolites in plasma of AIDS patients.: Beijnen et al. employed LiChrosorb® RP-8 in an HPLC method to quantify doxorubicin and its metabolites in plasma, offering a robust tool for therapeutic drug monitoring in AIDS patients (Beijnen et al., 1991).

Analysis Note

Pressure: ≤ 50 bar
Theoretical Plates (N/m) (Toluene): ≥ 55000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 0.5 - 1.5

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LiChrosorb is a registered trademark of Merck KGaA, Darmstadt, Germany


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