1.50435
SeQuant® ZIC®-HILIC 200Å (5 µm) HPLC Columns
L × I.D. 20 mm x 2.1 mm Guard Column
別名:
Hydrophilic Interaction Liquid Chromatography Guard Column
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この商品について
製品名
SeQuant® ZIC-HILIC™ Guard 20 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
material
PEEK-lined stainless steel column
Quality Segment
agency
suitable for USP L114
product line
SeQuant®
feature
endcapped: no
analyte functional class(es)
antibiotics, sweeteners, water soluble vitamins
analyte chemical class(es)
PFAS, alcohols / fermentation, aliphatic amines, alkaloids, amino acids, aromatic amines, carboxylic acids as methyl esters, esters, glycans, glycols, inorganic ions, monosaccharides, nitrosamines, nucleosides, oligonucleotides, organic phosphates, peptides, pesticides, phospholipids, polysaccharides, small carbohydrates, sugar alcohols, sulfonamides
parameter
550 bar pressure, 70 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)
column L × I.D.
2 cm × 2.1 mm
matrix
fully porous particle
matrix active group
ZIC-HILIC, ZIC-HILIC bonding phase
particle size
5 μm
pore size
200 Å pore size
operating pH
1.5-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
General description
SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility.
The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
Application
- Development and validation of LC-MS/MS methods to measure tobramycin and lincomycin: Demonstrates the application of SeQuant ZIC-HILIC Guard columns in a pharmacokinetic research study, focusing on the high-precision measurement of pharmaceutical compounds in biological matrices. This method is essential for ensuring accurate dosage and monitoring therapeutic levels, crucial for clinical trials and medical research (Pandey S et al., 2020).
Analysis Note
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