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Merck

16-202A

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate

clone JBW301, Upstate®, from mouse

別名:

H2AXS139P, Histone H2A.X (phospho S139), H2A histone family, member X, H2AX histone

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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製品名

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate, clone JBW301, Upstate®, from mouse

biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

JBW301, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable

isotype

IgG1

NCBI accession no.

NM_002105.2

UniProt accession no.

P16104

shipped in

dry ice

target post-translational modification

phosphorylation (pSer139)

Quality Level

100

Gene Information

human ... H2AX(3014)

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Analysis Note

Control
Staurosporine-treated Jurkat cells
Evaluated by Flow Cytometry to detect Log phase Jurkat cells treated with staurosporine.

Flow Cytometry: Log phase Jurkat cells were treated with staurosporine (1 μg/mL) for the indicated time. Histone H2A.X (Ser 139) phosphorylation was detected as described in the manual for the H2A.X Phosphorylation Assay Kit (Flow Cytometry), Catalog # 17-344. Cells were analyzed on a Becton-Dickinson FACS-Calibur flow cytometer.

Application

Immunocytochemistry: 2-4 μg/mL of a previous lot detected phospho-histone H2A.X in etoposide-treated HeLa cells fixed with 95% ethanol/5% acetic acid (10 minutes), followed by 1% formaldehyde, 0.25% Triton™ X-100 in TBS (5 min.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate (mouse monoclonal antibody) validated in FC, ICC to detect phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX histone.

Biochem/physiol Actions

Broad species cross-reactivity is expected based on conservation of sequence homology.
Recognizes Histone H2A.X phosphorylated at Ser139, MW 15 kDa.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
15 kDa

Immunogen

Epitope: Ser139
KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse. Clone JBW301.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified
Purified FITC-conjugated mouse monoclonal IgG1 in buffer containing PBS with 0.05% sodium azide. Frozen solution.

Preparation Note

Stable for 1 year at from date of receipt.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

試験成績書（COA）

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

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Lower phosphorylation of p38 MAPK blocks the oxidative stress-induced senescence in myeloid leukemic CD34(+)CD38 (-) cells.
Yin Xiao,Ping Zou,Jie Wang,Hui Song,Jing Zou,Lingbo Liu
Journal of Huazhong University of Science and Technology: Medical Sciences (Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban) null
An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans.
Ismail, IH; Wadhra, TI; Hammarsten, O
Nucleic Acids Research null
Protective role of Puralpha to cisplatin.
Kaminski, R; Darbinyan, A; Merabova, N; Deshmane, SL; White, MK; Khalili, K
Cancer Biology & Therapy null
Deqiang Ding et al.
Nature communications, 8(1), 819-819 (2017-10-12)
Piwi-interacting RNAs are small regulatory RNAs with key roles in transposon silencing and regulation of gametogenesis. The production of mature piwi-interacting RNAs requires a critical step of trimming piwi-interacting RNA intermediates to achieve optimally sized piwi-interacting RNAs. The poly(A)-specific ribonuclease
Uros Midic et al.
Molecular reproduction and development, 85(7), 635-648 (2018-06-15)
Structural maintenance of chromosome flexible domain containing 1 (Smchd1) is a chromatin regulatory gene for which mutations are associated with facioscapulohumeral muscular dystrophy and arhinia. The contribution of oocyte- and zygote-expressed SMCHD1 to early development was examined in mice (
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グローバルトレードアイテム番号

カタログ番号GTIN
16-202A04053252473616

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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