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Merck

17-10400

Anti-phospho-MLKL (Ser358) Antibody Set

from rabbit, purified by affinity chromatography

別名:

Mixed lineage kinase domain-like protein, MLKL

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human
Application:
WB
Citations:
6

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biological source

rabbit

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

western blot: suitable

UniProt accession no.

Q8NB16

shipped in

wet ice

target post-translational modification

phosphorylation (pSer358)

Quality Level

100

Gene Information

human ... MLKL(197259)

General description

Mixed lineage kinase domain-like protein (MLKL) is required for the execution of programmed necrosis. MLKL is a cell-permeable acrylamide compound that inhibits human, but not murine, MLKL adaptor function via covalent modification of Cys86. MLKL prevents the MLKL-RIP1-RIP3 necrosome complex from interacting with further downstream effectors.
This phospho-MLKL (Ser358) antibody set contains one vial of phospho-MLKL (Ser358) antibody and one vial of MLKL “Total” antibody.

RECOMMENDED USAGE:
It is recommended to first perform immunoprecipitation on your sample using the non-phosphorylated MLKL antibody for “total” MLKL protein isolation (see protocol section on page 2 of the Certificate of Analysis); then use the immunoprecipitated sample to detect the phosphorylated protein using the phospho-MLKL (Ser358) antibody via western blot.
~54 kDa

Immunogen

Epitope: Ser358
Linear peptide corresponding to the Protein Kinase Domain of phospho-MLKL (Ser358).

Application

Western Blot Analysis:
Untreated HT-29 cell lysates or lysates from HT-29 cells treated with Cycloheximide,Z-VAD-FMK and TNF alpha were first immunoprecipitated using the Anti-MLKL “total” antibody (1:1000 dilution) to isolate the MLKL protein.
The isolated “total” MLKL protein sample was then probed with Anti-phospho-MLKL (Ser358) (1:1000 dilution).
Anti-phospho-MLKL (Ser358) Antibody Set is an antibody set against phospho-MLKL (Ser358) for use in WB.

Biochem/physiol Actions

When used together and under the “Recommended Usage” instructions, this antibody set will detect MLKL phosphorylated at Ser358.

Physical form

100 µL of Anti-phospho-MLKL (Ser358) purified polyclonal antibody in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.

100 µg (concentration: 1 mg/mL) of Anti- MLKL purified polyclonal antibody in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.

Analysis Note

Anti- MLKL (Total Antibody): Evaluated by Immunoprecipitation on HT-29 cell lysates.

Anti-phospho-MLKL (Ser358) Antibody: Evaluated by Dot Blot on phosphorylated and non-phosphorylated MLKL peptides.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
One vial of phospho-MLKL (Ser358) antibody

One vial of MLKL “Total” antibody

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保管分類

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

17-10400:

jan

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Haley M Hardin et al.
Molecular cancer therapeutics, 22(11), 1280-1289 (2023-08-01)
Neurofibromatosis Type 2 (NF2) is a tumor predisposition syndrome caused by germline inactivating mutations in the NF2 gene encoding the merlin tumor suppressor. Patients develop multiple benign tumor types in the nervous system including bilateral vestibular schwannomas (VS). Standard treatments
Abdulrahman Allaf et al.
Cold Spring Harbor molecular case studies, 8(4) (2022-06-23)
Schwannomatosis is a rare genetic disorder that predisposes individuals to development of multiple schwannomas mainly in spinal and peripheral nerves and to debilitating chronic pain often unrelated to any schwannoma. Pathogenic variants of two genes, SMARCB1 and LZTR1, are causal
Shrikant R Mulay et al.
Nature communications, 7, 10274-10274 (2016-01-29)
Crystals cause injury in numerous disorders, and induce inflammation via the NLRP3 inflammasome, however, it remains unclear how crystals induce cell death. Here we report that crystals of calcium oxalate, monosodium urate, calcium pyrophosphate dihydrate and cystine trigger caspase-independent cell
Contraction Band Necrosis with Dephosphorylated Connexin 43 in Rat Myocardium after Daily Cocaine Administration.
Wen, et al.
International Journal of Molecular Sciences, 23 (2022)
Qiong Wang et al.
Translational oncology, 13(2), 372-382 (2019-12-31)
The efficacy of chemotherapeutic agents in killing cancer cells is mainly attributed to the induction of apoptosis. However, the tremendous efforts on enhancing apoptosis-related mechanisms have only moderately improved lung cancer chemotherapy, suggesting that other cell death mechanisms such as
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グローバルトレードアイテム番号

カタログ番号GTIN
17-1040004053252934599

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