189737
Streptavidin, Phycoerythrin Conjugate
This Streptavidin, Phycoerythrin Conjugate is validated for use in Enzyme Immunoassay, Flow Cytometry.
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この商品について
NACRES:
NA.41
UNSPSC Code:
41116133
Form:
liquid
biological source
(Streptomyces avidinii)
Quality Segment
form
liquid
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
do not freeze, protect from light
dilution
(Enzyme Immunoassay
Flow Cytometry (1-3 µg per 1 x 10 cells in 100 µL))
fluorescence
λex 500 nm, λem 575 nm
shipped in
ambient
storage temp.
2-8°C
General description
Streptavidin conjugated to R-phycoerythrin. Specific for biotin, biotinylated proteins, or biotinylated antibodies.
Application
Enzyme Immunoassay (see comments)
Flow Cytometry (1-3 µg per 1 x 10⁶ cells in 100 µl)
Flow Cytometry (1-3 µg per 1 x 10⁶ cells in 100 µl)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 50 mM sodium phosphate, 1% BSA, 0.1% glycerol, pH 7.6. Ammonium sulfate free.
Preparation Note
Do not dilute the entire vial at one time. Dilute only as needed in 50 mM sodium phosphate buffer, pH 7.2, 2-5 mg/ml of BSA or other physiological buffer.
Other Notes
Lindmo, T., et al. 1990. J. Immunol. Methods126, 183.
Starling, J.J., et al. 1988. Cancer Res.48, 6211.
Starling, J.J., et al. 1988. Cancer Res.48, 6211.
The phycobiliprotein used in making this conjugate had a M.W. of 240,000. Molar extinction coefficient at 565 nm: 1.9 x 106. Excitation Max: 500 nm emission max: 575 nm.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
保管分類
12 - Non Combustible Liquids
wgk
WGK 2