biological source
(Synthetic chemical)
Quality Segment
form
solution
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
clearvery pale yellow
shipped in
ambient
storage temp.
2-8°C
General description
Peroxidase substrate that is soluble, safe, convenient and has good overall performance in ELISA. ABTS™ Chromophore develops intense blue-green color that can be measured at 405 nm to 410 nm. May be used for both kinetic and endpoint reactions.
Single component peroxidase substrate which is soluble, safe, convenient and has good overall performance for ELISA. In the presence of horseradish peroxidase (HRP) and hydrogen peroxide, ABTS is oxidized to an intense blue-green, water-soluble product. This product is a radical cation having an absorption maximum at 416 nm. Typically, product formation is measured spectrophotometrically at 405 nm, allowing a quantitative analysis of HRP-based systems. The color formation as a function of time may be monitored, or the reaction may be stopped by the addition of acid; since the acid does not alter the absorbance at 405 nm, one may perform both kinetic and endpoint measurements at the same wavelength.
Physical form
Contains optimal concentrations of ABTS and H₂O₂ in a citrate buffer (pH 4.0) with proprietary stabilizers.
Preparation Note
ABTS™ solution should be stored at 4°C, but must be warmed to room temperature before use. The solution should be protected from exposure to direct sunlight, and should be discarded if it becomes bright yellow or turbid.
Other Notes
Rodgriquez-Lopez, J.N., et al. 2000. Biochemistry39, 13201.
Legal Information
ABTS is a trademark of Boehringer Mannheim Corp.
ABTS is a trademark of Roche
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
保管分類
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Substances Subject to be Notified Names
ishl_notified
194434-ML: + 194434-100ML:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。