203390
(±)-Blebbistatin
≥97% (HPLC), solid, Myosin II inhibitor, Calbiochem®
別名:
(±)-Blebbistatin
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この商品について
実験式（ヒル表記法）:
C18H16N2O2
CAS番号:
分子量:
292.33
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77
Assay:
≥97% (HPLC)
Form:
solid
Quality level:
Storage condition:
OK to freeze, protect from light
製品名
(±)-Blebbistatin, (±)-Blebbistatin, CAS 674289-55-5, is a cell-permeable, selective, and reversible inhibitor of nonmuscle myosin II. Blocks cell blebbing and disrupts cell migration & cytoKinesis in vertebrate cells.
SMILES string
N2(CCC3(C2=Nc4c(cc(cc4)C)C3=O)O)c1ccccc1
InChI
1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8,11,22H,9-10H2,1H3
InChI key
LZAXPYOBKSJSEX-UHFFFAOYSA-N
assay
≥97% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
yellow
solubility
methanol: 1.5 mg/mL, 100% DMSO: 100 mg/mL, 90% DMSO: 75 mg/mL
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of nonmuscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤ 100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly. A 50 mM (5 mg/342 µl) solution of (±)-Blebbistatin (Cat. No. 203389 ) in 90% DMSO is also available.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
ATPase
ATPase
Product does not compete with ATP.
Reversible: yes
Target IC50: <100 µM inhibiting the ATPase and gliding motility of human platelets
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution in 90% DMSO, aliquot and freeze (-20°C). Stock solutions in 90% DMSO are stable for up to 1 month at -20°C. Stock solutions in 100% DMSO are unstable; reconstitute just prior to use.
Other Notes
Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003. Science299, 1743.
Cheung, A., et al. 2001. Mol. Biol. Cell Suppl.12, 271a.
Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003. Science299, 1743.
Cheung, A., et al. 2001. Mol. Biol. Cell Suppl.12, 271a.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
203390-5MG: + 203390-VMG: + 203390-1.1ML: + 203390-5UNMG:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 203390-5MGCN | 04055977221268 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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