203980
α-Bungarotoxin
from Bungarus multicinctus, lyophilized solid, irreversible binder to motor end-plate acetylcholine receptor, Calbiochem®
別名:
α-Bungarotoxin, Bungarus multicinctus
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この商品について
実験式（ヒル表記法）:
C338H529N97O105S11
CAS番号:
分子量:
7984.12
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77
Form:
lyophilized solid
Storage condition:
OK to freeze
製品名
α-Bungarotoxin, Bungarus multicinctus, from Bungarus multicinctus, lyophilized solid, irreversible binder to motor end-plate acetylcholine receptor, Calbiochem®
Quality Segment
description
Merck USA index - 14, 1488
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
95% (HPLC)
solubility
PBS: soluble, water: soluble
shipped in
ambient
storage temp.
−20°C
General description
A polypeptide composed of 74 amino acids containing 5 disulfide bridges. Blocks neuromuscular transmission by irreversible binding to the motor end-plate acetylcholine receptor (Kd = 1 nM to 1 pM) but does not depress acetylcholine release from motor nerve endings. Blocks nicotine-induced augmentation in intracellular Ca2+ in PC12 cells (IC50 = 310 nM). Prevents opening of nicotinic receptor-associated ion channels. Reconstitution experiments in Xenopus oocytes have shown the effects of α-Bungarotoxin on neuronal nAChR to be highly specific for the α7-subtype (IC50 = 1.6 nM), but not for the α3β4-subtype (IC50 >3 µM).
Blocks neuromuscular transmission by irreversible binding to motor end-plate acetylcholine receptor (Kd = 1 pM to 1 nM) but does not depress acetylcholine release from motor nerve endings. Blocks nicotine-induced increase of intracellular Ca2+ in PC12 cells (IC50 = 310 nM), and prevents opening of nicotinic receptor-associated ion channels. Reconstitution experiments in Xenopus oocytes have shown the effects of α-bungarotoxin on neuronal nAChR to be highly specific for the α7-subtype (IC50 = 1.6 nM), but not for the α3β4-subtype (IC50 >3 µM).
Biochem/physiol Actions
Cell permeable: no
Kd = 1 pM to 1 nM for motor end-plate acetylcholine receptor
Primary Target
Motor end-plate acetylcholine receptor
Motor end-plate acetylcholine receptor
Product does not compete with ATP.
Reversible: no
Physical form
Supplied as an acetate salt
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
H-Ile-Val-Cys³-His-Thr-Thr-Ala-Thr-Ser-Pro-Ile-Ser-Ala-Val-Thr-Cys¹⁶-Pro-Pro-Gly-Glu-Asn-Leu-Cys²³-Tyr-Arg-Lys-Met-Trp-Cys²⁹-Asp-Ala-Phe-Cys³³-Ser-Ser-Arg-Gly-Lys-Val-Val-Glu-Leu-Gly-Cys⁴⁴-Ala-Ala-Thr-Cys⁴⁸-Pro-Ser-Lys-Lys-Pro-Tyr-Glu-Glu-Val-Thr-Cys⁵⁹-Cys⁶⁰-Ser-Thr-Asp-Lys-Cys⁶⁵-Asn-Pro-His-Pro-Lys-Gln-Arg-Pro-Gly-OH (disulfide bonds: 3 → 23; 16 → 44; 29 → 33; 48 → 59; 60 → 65)
Lopez, M.G., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 14184.
Zhang, Z.W., et al. 1994. Neuron 12, 167.
Bambrick, L.L., and Gordon, T. 1992. J. Physiol.449, 479.
Lin, S.R., and Chang, C.C. 1992. Biochim. Biophys. Acta1159, 255.
Motomura, M., et al. 1992. Neurosci. Lett.143, 139.
Sorenson, E.M., and Chiappinelli, V.A. 1992. J. Comp. Neurol.323, 1.
Ruan, K.H., et al. 1990. Proc. Natl. Acad. Sci. USA87, 6156.
Zhang, Z.W., et al. 1994. Neuron 12, 167.
Bambrick, L.L., and Gordon, T. 1992. J. Physiol.449, 479.
Lin, S.R., and Chang, C.C. 1992. Biochim. Biophys. Acta1159, 255.
Motomura, M., et al. 1992. Neurosci. Lett.143, 139.
Sorenson, E.M., and Chiappinelli, V.A. 1992. J. Comp. Neurol.323, 1.
Ruan, K.H., et al. 1990. Proc. Natl. Acad. Sci. USA87, 6156.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable