324717
Endoglycosidase H
Cleaves high-mannose N-glycans, suitable for Mass Spectrometry, from Streptomyces plicatus, recombinant, expressed in E. coli
別名:
Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli, Endo-β-N-acetylglucosaminidase H, Endo H
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この商品について
CAS番号:
MDL number:
NACRES:
NA.54
UNSPSC Code:
12352202
Specific activity:
≥3 units/mL, ≥30 units/mg protein
Recombinant:
expressed in E. coli
製品名
Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli, Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.
recombinant
expressed in E. coli
Quality Segment
conjugate
(N-linked)
form
liquid
specific activity
≥3 units/mL, ≥30 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
foreign activity
N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases, none detected
shipped in
wet ice
storage temp.
2-8°C
General description
Note: 1 mU = 1 milliunit.
Recombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.
Physical form
In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
Other Notes
One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.
Rao, V., et al. 1995. Structure 3, 449.
Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
Trumbly, R.J., et al. 1985. J. Biol. Chem.260, 5683.
Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
Trumbly, R.J., et al. 1985. J. Biol. Chem.260, 5683.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
保管分類
12 - Non Combustible Liquids
wgk
WGK 2
試験成績書（COA）
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