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324725

Endoglycosidase F1

Name: Endoglycosidase F1
Brand: MM

Cleaves specific N-glycan structures, suitable for Mass Spectrometry, from Elizabethkingia meningosepticum, recombinant, expressed in E. coli

別名:

Endo-β-N-acetylglucosaminidase F1, Endo F1

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この商品について

EC Number:

3.2.1.96 (BRENDA, IUBMB)

UNSPSC Code:

12352202

NACRES:

NA.54

Specific activity:

≥16 units/mg protein, ≥17 units/mL

Recombinant:

expressed in E. coli

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特性

製品名

Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli, Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and hybrid. Suitable for deglycosylation of native proteins.

recombinant

expressed in E. coli

Quality Segment

100

conjugate

(N-linked)

form

liquid

specific activity

≥16 units/mg protein, ≥17 units/mL

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

technique(s)

protein mass spectrometry: suitable

foreign activity

Proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

詳細

General description

Note: 1 mU = 1 milliunit.

Recombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins.

Physical form

In 20 mM Tris-HCl, pH 7.5.

Other Notes

One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5.

Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Tarentino, A.L., et al. 1992. J. Biol. Chem. 267, 3868.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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保管分類

10 - Combustible liquids

一体何が起きているの？

WGK 1

引火点 (F)

Not applicable

引火点（°C）