324831
EGF human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, solid, suitable for cell culture
別名:
EGF, Human, Recombinant, E. coli, Epidermal Growth Factor, Human, Recombinant, E. coli
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この商品について
Recombinant:
expressed in E. coli
Assay:
≥95% (SDS-PAGE)
Form:
solid
Impurities:
<1.0 EU/μg Endotoxin (EU/μg EGF)
製品名
EGF, Human, Recombinant, E. coli,
recombinant
expressed in E. coli
description
Merck USA index - 14, 3527
assay
≥95% (SDS-PAGE)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<1.0 EU/μg Endotoxin (EU/μg EGF)
shipped in
ambient
storage temp.
−20°C
Quality Level
Biochem/physiol Actions
ED₅₀ = 100-400 pg/ml as measured by its ability to stimulate the ³H-thymidine incorporation in an EGF-responsive mouse fibroblast cell line, Balb/3T3.
Disclaimer
Toxicity: Standard Handling (A)
General description
Recombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to native human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Binding of EGF to its 170 kDa transmembrane receptor results in dimerization of the receptor, activation of the intrinsic receptor tyrosine kinase, and initiation of the complex signal transduction cascade that leads to cell proliferation and/or differentiation. Acts as a survival factor in preventing apoptosis.
Other Notes
Merlo, G.R., et al. 1995. J. Cell Biol. 128, 1185.
Yoshida, T., et al. 1993. Brain Res. Dev.76, 147.
Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA88, 415.
Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69.
Downward, J., et al. 1984. Nature311, 483.
Gray, A., et al. 1983. Nature303, 722.
Scott, J., et al. 1983. Science221, 236.
Cohen, S. 1960. Proc. Natl. Acad. Sci. USA46, 302.
Yoshida, T., et al. 1993. Brain Res. Dev.76, 147.
Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA88, 415.
Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69.
Downward, J., et al. 1984. Nature311, 483.
Gray, A., et al. 1983. Nature303, 722.
Scott, J., et al. 1983. Science221, 236.
Cohen, S. 1960. Proc. Natl. Acad. Sci. USA46, 302.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C.
Reconstitute to a concentration of ≥10 µg/ml in 10 mM acetic acid containing ≥0.1% HSA or BSA.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類
11 - Combustible Solids
wgk
WGK 2
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
324831-0MG: + 324831-1MG: + 324831-UG: + 324831-200UG:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Eric Okerberg et al.
Current protocols in chemical biology, 5(3), 213-226 (2014-01-07)
The protocols in this unit describe efficient and cost-effective approaches to determine the interaction of small-molecule inhibitors with native kinases, and also analyze the interactions between kinases and their binding partners in a cellular setting. The combined attributes of activity-based
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In response to estrogens, estrogen receptor alpha (ERα), a critical regulator of homeostasis, is degraded through the 26S proteasome. However, despite the continued presence of estrogen before menopause, ERα protein levels are maintained. We discovered that ERK1/2-RSK2 activity oscillates during
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The Journal of neuroscience : the official journal of the Society for Neuroscience, 41(13), 2899-2910 (2021-02-28)
The addition of new neurons to existing neural circuits in the adult brain remains of great interest to neurobiology because of its therapeutic implications. The premier model for studying this process has been the hippocampal dentate gyrus in mice, where
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Developmental cell, 55(5), 558-573 (2020-10-16)
DNA crosslinking agents are commonly used in cancer chemotherapy; however, responses of normal tissues to these agents have not been widely investigated. We reveal in mouse interfollicular epidermal, mammary and hair follicle epithelia that genotoxicity does not promote apoptosis but
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 324831-200UGCN | 04055977216189 |
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