Human Fibronectin

Fibronectin is a glycoprotein belonging to the extracellular matrix (ECM) protein family. Fibronectin contains two polypeptides (α and β chains) linked by two disulfide bonds near the carboxyl termini to form a dimer.Native fibronectin purified from human plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.

The human plasma fibronectin has been used for coating surfaces such as poly-l-lysine (PLL)-coated nanochips, polyacrylamide (PAAm) hydrogel substrates, glass slides and more, providing a substrate for the adhesion and culture of cells in various experimental setups.

Human fibronectin protein plays crucial roles in various cellular behaviors such as cell attachment, migration, embryogenesis, wound healing, tissue integrity, and malignant processes. One of the key features enabling fibronectin to support cell adhesion is the presence of the Arg-Gly-Asp (RGD) tripeptide. It has been extensively studied in areas like fibronectin coating on polystyrene (PS) and poly(lactic-co-glycolic acid) (PLGA) surfaces. Additionally, fibronectin has been the subject of significant research regarding its involvement in regulating processes such as differentiation-to-growth switching, tubulin monomer growth, cell cycle status, cell spreading, and motility.

Please refer to vial label for lot-specific concentration.

In PBS, pH 7.3.

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Thaw carefully using the guidelines outlined above. Following initial thaw, aliquot and freeze (-20°C).

Contaminants: none detected by IEP with antiserum to fibronectin or normal human serum

single major band by SDS-PAGE

Akiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol.59, 1.
Krejci, K., and Fritz, H. 1976. FEBS Lett.64, 152.


Selected Citations
Hoffmeister, M., et al. 2008. J. Biol. Chem.283, 2297.


Brown, S., et al. 2002. Nature418, 200.


Kim, M., FEBS Letters581 1917.

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Toxicity: Standard Handling (A)